Direct heterogeneous electron transfer reactions of Trametes hirsuta Laccase at bare and thiol-modified gold electrodes
(2006) In Electroanalysis 18(19-20). p.1901-1908- Abstract
- Direct electron transfer reactions of high redox potential Trametes hirsuta laccase on bare and 15 different thiol-modified gold electrodes were studied using cyclic voltammetry and potentiometry: Well-pronounced Faradaic processes were obtained for the enzyme adsorbed on bare gold, whereas reproducible and stable electrochemistry was obtained only when 4-aminothiophenol was used for gold modification. Moreover, the laccase-4-aminothiophenol-modified gold electrode showed the highest value of the steady-state potential under aerobic conditions equal to 660 mV vs. NHE compared with the other 15 different thiol modified electrodes and also the bare electrodes with immobilized enzyme. However, this value is still too far away from the... (More)
- Direct electron transfer reactions of high redox potential Trametes hirsuta laccase on bare and 15 different thiol-modified gold electrodes were studied using cyclic voltammetry and potentiometry: Well-pronounced Faradaic processes were obtained for the enzyme adsorbed on bare gold, whereas reproducible and stable electrochemistry was obtained only when 4-aminothiophenol was used for gold modification. Moreover, the laccase-4-aminothiophenol-modified gold electrode showed the highest value of the steady-state potential under aerobic conditions equal to 660 mV vs. NHE compared with the other 15 different thiol modified electrodes and also the bare electrodes with immobilized enzyme. However, this value is still too far away from the equilibrium potential of the oxygen electrode and Trametes hirsuta laccase-modified graphite electrode, for which a well-pronounced high potential process of oxygen bioelectroreduction is shown at 800 mV vs. NHE. Possible mechanisms of the enzyme function on bare and thiol-modified gold electrodes are discussed in correlation with the structure and orientation of the enzyme on the surface of the various electrodes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/384112
- author
- Shleev, Sergey LU ; Pita, Marcos ; Yaropolov, Alexander ; Ruzgas, Tautgirdas LU and Gorton, Lo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- redox potential, and T3 sites, T2, laccase, T1, gold electrode
- in
- Electroanalysis
- volume
- 18
- issue
- 19-20
- pages
- 1901 - 1908
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000241621300007
- scopus:33750470362
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.200603600
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- af3c131b-29da-4206-9db5-00efe733f935 (old id 384112)
- date added to LUP
- 2016-04-01 16:43:39
- date last changed
- 2022-02-20 08:04:50
@article{af3c131b-29da-4206-9db5-00efe733f935,
abstract = {{Direct electron transfer reactions of high redox potential Trametes hirsuta laccase on bare and 15 different thiol-modified gold electrodes were studied using cyclic voltammetry and potentiometry: Well-pronounced Faradaic processes were obtained for the enzyme adsorbed on bare gold, whereas reproducible and stable electrochemistry was obtained only when 4-aminothiophenol was used for gold modification. Moreover, the laccase-4-aminothiophenol-modified gold electrode showed the highest value of the steady-state potential under aerobic conditions equal to 660 mV vs. NHE compared with the other 15 different thiol modified electrodes and also the bare electrodes with immobilized enzyme. However, this value is still too far away from the equilibrium potential of the oxygen electrode and Trametes hirsuta laccase-modified graphite electrode, for which a well-pronounced high potential process of oxygen bioelectroreduction is shown at 800 mV vs. NHE. Possible mechanisms of the enzyme function on bare and thiol-modified gold electrodes are discussed in correlation with the structure and orientation of the enzyme on the surface of the various electrodes.}},
author = {{Shleev, Sergey and Pita, Marcos and Yaropolov, Alexander and Ruzgas, Tautgirdas and Gorton, Lo}},
issn = {{1040-0397}},
keywords = {{redox potential; and T3 sites; T2; laccase; T1; gold electrode}},
language = {{eng}},
number = {{19-20}},
pages = {{1901--1908}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electroanalysis}},
title = {{Direct heterogeneous electron transfer reactions of Trametes hirsuta Laccase at bare and thiol-modified gold electrodes}},
url = {{http://dx.doi.org/10.1002/elan.200603600}},
doi = {{10.1002/elan.200603600}},
volume = {{18}},
year = {{2006}},
}