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Sterols of the Green-Pigmented, Freshwater Raphidophyte, Gonyostomum semen, from Scandinavian Lakes

Leblond, Jeffrey D.; Dahmen, Aaron S.; Lebret, Karen LU and Rengefors, Karin LU (2013) In Journal of Eukaryotic Microbiology 60(4). p.399-405
Abstract
Sterols are a class of membrane-reinforcing, ringed lipids which have a long history of examination in algae as a means of deriving chemotaxonomic relationships and as potential lipidic biomarkers. The Raphidophyceae represent a class of harmful, bloom-forming, marine and freshwater algae. To date, there have been four published examinations of their sterol composition, focusing primarily on brown-pigmented, marine species within the genera, Chattonella, Fibrocapsa, and Heterosigma. Lacking in these examinations has been the species Gonyostomum semen Ehrenb., which is a green-pigmented, freshwater raphidophyte with a worldwide distribution. The goal of this study was to examine the sterol composition of this nuisance alga, determine the... (More)
Sterols are a class of membrane-reinforcing, ringed lipids which have a long history of examination in algae as a means of deriving chemotaxonomic relationships and as potential lipidic biomarkers. The Raphidophyceae represent a class of harmful, bloom-forming, marine and freshwater algae. To date, there have been four published examinations of their sterol composition, focusing primarily on brown-pigmented, marine species within the genera, Chattonella, Fibrocapsa, and Heterosigma. Lacking in these examinations has been the species Gonyostomum semen Ehrenb., which is a green-pigmented, freshwater raphidophyte with a worldwide distribution. The goal of this study was to examine the sterol composition of this nuisance alga, determine the potential of using its sterol profile as a biomarker, and finally to determine if there is any intraspecific variability between isolates. We have examined 21 isolates of G. semen from a number of Scandinavian lakes, and all were found to produce two major sterols, 24-ethylcholesta-5,22E-dien-3-ol and 24-ethylcholest-5-en-3-ol, and 24-methylcholest-5-en-3-ol as a minor sterol; the presence of 24-ethylcholesta-5,22E-dien-3-ol differentiates G. semen from brown-pigmented, marine raphidophytes which generally lack it. The results of this study indicate that isolates of G. semen from geographically separate lakes across Finland and Scandinavia have the same sterol biosynthetic pathway, and that there is no evolutionary divergence between the isolates with regard to sterol composition. The sterols of G. semen are not considered to be useful biomarkers for this particular organism because they are commonly found in other algae and plants. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lipid, Raphidophyceae, sterol
in
Journal of Eukaryotic Microbiology
volume
60
issue
4
pages
399 - 405
publisher
Wiley-Blackwell
external identifiers
  • wos:000321182900008
  • scopus:84879798548
ISSN
1066-5234
DOI
10.1111/jeu.12050
language
English
LU publication?
yes
id
af40a5a2-0a97-4f59-a34c-b457eef382f9 (old id 3979700)
date added to LUP
2013-09-06 16:18:02
date last changed
2019-02-20 06:10:07
@article{af40a5a2-0a97-4f59-a34c-b457eef382f9,
  abstract     = {Sterols are a class of membrane-reinforcing, ringed lipids which have a long history of examination in algae as a means of deriving chemotaxonomic relationships and as potential lipidic biomarkers. The Raphidophyceae represent a class of harmful, bloom-forming, marine and freshwater algae. To date, there have been four published examinations of their sterol composition, focusing primarily on brown-pigmented, marine species within the genera, Chattonella, Fibrocapsa, and Heterosigma. Lacking in these examinations has been the species Gonyostomum semen Ehrenb., which is a green-pigmented, freshwater raphidophyte with a worldwide distribution. The goal of this study was to examine the sterol composition of this nuisance alga, determine the potential of using its sterol profile as a biomarker, and finally to determine if there is any intraspecific variability between isolates. We have examined 21 isolates of G. semen from a number of Scandinavian lakes, and all were found to produce two major sterols, 24-ethylcholesta-5,22E-dien-3-ol and 24-ethylcholest-5-en-3-ol, and 24-methylcholest-5-en-3-ol as a minor sterol; the presence of 24-ethylcholesta-5,22E-dien-3-ol differentiates G. semen from brown-pigmented, marine raphidophytes which generally lack it. The results of this study indicate that isolates of G. semen from geographically separate lakes across Finland and Scandinavia have the same sterol biosynthetic pathway, and that there is no evolutionary divergence between the isolates with regard to sterol composition. The sterols of G. semen are not considered to be useful biomarkers for this particular organism because they are commonly found in other algae and plants.},
  author       = {Leblond, Jeffrey D. and Dahmen, Aaron S. and Lebret, Karen and Rengefors, Karin},
  issn         = {1066-5234},
  keyword      = {Lipid,Raphidophyceae,sterol},
  language     = {eng},
  number       = {4},
  pages        = {399--405},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Eukaryotic Microbiology},
  title        = {Sterols of the Green-Pigmented, Freshwater Raphidophyte, Gonyostomum semen, from Scandinavian Lakes},
  url          = {http://dx.doi.org/10.1111/jeu.12050},
  volume       = {60},
  year         = {2013},
}