Quantitation of J chain in human biological fluids by a simple immunochemical procedure
Grubb, Anders O LU
(1978)
In Acta Medica Scandinavica
204(6).
p.65-453
- Abstract
The molecular form and immunochemical properties of the J chain populations released on reduction and carboxymethylation of normal human plasma, milk, saliva and of plasma containing IgA or IgM M-components were investigated. A procedure was devised to release the entire J chain population from these various sources and to produce immunochemically identical J chain populations containing only J chain monomers. An identical standard J chain population was purified and quantitated by physiochemical means. A specific rabbit anti-J chain antiserum was raised against this pure J chain population. A simple and rapid immunochemical method for J chain quantitation in complex biological fluids as well as in solutions of pure polymeric... (More)
The molecular form and immunochemical properties of the J chain populations released on reduction and carboxymethylation of normal human plasma, milk, saliva and of plasma containing IgA or IgM M-components were investigated. A procedure was devised to release the entire J chain population from these various sources and to produce immunochemically identical J chain populations containing only J chain monomers. An identical standard J chain population was purified and quantitated by physiochemical means. A specific rabbit anti-J chain antiserum was raised against this pure J chain population. A simple and rapid immunochemical method for J chain quantitation in complex biological fluids as well as in solutions of pure polymeric immunoglobulins was constructed on these grounds. The J chain concentration was found to be (mean +/- S.D.) 1.74 +/- 0.65 micron in normal human plasma, 1.94 +/- 1.21 micron in human milk and 0.48 +/- 0.26 micron in human saliva. The J chain/IgA molar ratio was found to be (mean +/- S.D.) 0.45 +/- 0.07 in human milk and 0.52 +/- 0.09 in human saliva when the IgA concentration was expressed as monomeric units per volume unit. The range of the J chain/IgA molar ratios in plasma samples with highly concentrated IgA M-components was 0-0.64. The J chain/IgM molar ratio in plasma samples with highly concentrated IgM M-components was between 1 and 2 when the IgM concentration was expressed as pentameric units per volume unit.
(Less)
- author
- Grubb, Anders O
LU
- organization
- publishing date
- 1978
- type
- Contribution to journal
- publication status
- published
- keywords
- Amino Acids/analysis, Chromatography, Gel, Electrophoresis, Agar Gel, Electrophoresis, Polyacrylamide Gel, Female, Humans, Immune Sera, Immunodiffusion, Immunoelectrophoresis, Immunoelectrophoresis, Two-Dimensional, Immunoglobulin A/analysis, Immunoglobulin J-Chains/analysis, Immunoglobulin M/analysis, Immunologic Techniques, Milk, Human/immunology, Saliva/immunology
- in
- Acta Medica Scandinavica
- volume
- 204
- issue
- 6
- pages
- 65 - 453
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0018223360
- pmid:104551
- ISSN
- 0001-6101
- DOI
- 10.1111/j.0954-6820.1978.tb08473.x
- language
- English
- LU publication?
- yes
- id
- afe18a49-31d6-42bb-8050-a042f3d86b0c
- date added to LUP
- 2021-10-16 19:25:33
- date last changed
- 2024-01-12 02:58:40
@article{afe18a49-31d6-42bb-8050-a042f3d86b0c,
abstract = {{<p>The molecular form and immunochemical properties of the J chain populations released on reduction and carboxymethylation of normal human plasma, milk, saliva and of plasma containing IgA or IgM M-components were investigated. A procedure was devised to release the entire J chain population from these various sources and to produce immunochemically identical J chain populations containing only J chain monomers. An identical standard J chain population was purified and quantitated by physiochemical means. A specific rabbit anti-J chain antiserum was raised against this pure J chain population. A simple and rapid immunochemical method for J chain quantitation in complex biological fluids as well as in solutions of pure polymeric immunoglobulins was constructed on these grounds. The J chain concentration was found to be (mean +/- S.D.) 1.74 +/- 0.65 micron in normal human plasma, 1.94 +/- 1.21 micron in human milk and 0.48 +/- 0.26 micron in human saliva. The J chain/IgA molar ratio was found to be (mean +/- S.D.) 0.45 +/- 0.07 in human milk and 0.52 +/- 0.09 in human saliva when the IgA concentration was expressed as monomeric units per volume unit. The range of the J chain/IgA molar ratios in plasma samples with highly concentrated IgA M-components was 0-0.64. The J chain/IgM molar ratio in plasma samples with highly concentrated IgM M-components was between 1 and 2 when the IgM concentration was expressed as pentameric units per volume unit.</p>}},
author = {{Grubb, Anders O}},
issn = {{0001-6101}},
keywords = {{Amino Acids/analysis; Chromatography, Gel; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Female; Humans; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Immunoelectrophoresis, Two-Dimensional; Immunoglobulin A/analysis; Immunoglobulin J-Chains/analysis; Immunoglobulin M/analysis; Immunologic Techniques; Milk, Human/immunology; Saliva/immunology}},
language = {{eng}},
number = {{6}},
pages = {{65--453}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Medica Scandinavica}},
title = {{Quantitation of J chain in human biological fluids by a simple immunochemical procedure}},
url = {{http://dx.doi.org/10.1111/j.0954-6820.1978.tb08473.x}},
doi = {{10.1111/j.0954-6820.1978.tb08473.x}},
volume = {{204}},
year = {{1978}},
}