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IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT) : Its validation and use

Schellenberg, François; Wielders, Jos; Anton, Raymond; Bianchi, Vincenza; Deenmamode, Jean; Weykamp, Cas; Whitfield, John B; Jeppsson, Jan Olof LU and Helander, Anders (2017) In Clinica Chimica Acta 465. p.91-100
Abstract

Carbohydrate-deficient transferrin (CDT) is used as a biomarker of sustained high alcohol consumption. The currently available measurement procedures for CDT are based on various analytical techniques (HPLC, capillary electrophoresis, nephelometry), some differing in the definition of the analyte and using different reference intervals and cut-off values. The Working Group on Standardization of CDT (WG-CDT), initiated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), has validated an HPLC candidate reference measurement procedure (cRMP) for CDT (% disialotransferrin to total transferrin based on peak areas), demonstrating that it is suitable as a reference measurement procedure (RMP) for CDT.... (More)

Carbohydrate-deficient transferrin (CDT) is used as a biomarker of sustained high alcohol consumption. The currently available measurement procedures for CDT are based on various analytical techniques (HPLC, capillary electrophoresis, nephelometry), some differing in the definition of the analyte and using different reference intervals and cut-off values. The Working Group on Standardization of CDT (WG-CDT), initiated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), has validated an HPLC candidate reference measurement procedure (cRMP) for CDT (% disialotransferrin to total transferrin based on peak areas), demonstrating that it is suitable as a reference measurement procedure (RMP) for CDT. Presented is a detailed description of the cRMP and its calibration. Practical aspects on how to treat genetic variant and so-called di-tri bridge samples are described. Results of method performance characteristics, as demanded by ISO 15189 and ISO 15193, are given, as well as the reference interval and measurement uncertainty and how to deal with that in routine use. The correlation of the cRMP with commercial CDT procedures and the performance of the cRMP in a network of laboratories are also presented. The performance of the CDT cRMP in combination with previously developed commutable calibrators allows for standardization of the currently available commercial measurement procedures for CDT. The cRMP has recently been approved by the IFCC and will be from now on be known as the IFCC-RMP for CDT, while CDT results standardized according to this RMP should be indicated as CDTIFCC.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcohol biomarker, CDT, Disialotransferrin, HPLC, Reference Measurement Procedure (RMP), Standardization
in
Clinica Chimica Acta
volume
465
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85007421349
ISSN
0009-8981
DOI
10.1016/j.cca.2016.12.022
language
English
LU publication?
no
id
b01d6f5f-909d-425f-a27c-6ecf928e4472
date added to LUP
2017-02-06 08:52:59
date last changed
2018-01-21 04:19:12
@article{b01d6f5f-909d-425f-a27c-6ecf928e4472,
  abstract     = {<p>Carbohydrate-deficient transferrin (CDT) is used as a biomarker of sustained high alcohol consumption. The currently available measurement procedures for CDT are based on various analytical techniques (HPLC, capillary electrophoresis, nephelometry), some differing in the definition of the analyte and using different reference intervals and cut-off values. The Working Group on Standardization of CDT (WG-CDT), initiated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC), has validated an HPLC candidate reference measurement procedure (cRMP) for CDT (% disialotransferrin to total transferrin based on peak areas), demonstrating that it is suitable as a reference measurement procedure (RMP) for CDT. Presented is a detailed description of the cRMP and its calibration. Practical aspects on how to treat genetic variant and so-called di-tri bridge samples are described. Results of method performance characteristics, as demanded by ISO 15189 and ISO 15193, are given, as well as the reference interval and measurement uncertainty and how to deal with that in routine use. The correlation of the cRMP with commercial CDT procedures and the performance of the cRMP in a network of laboratories are also presented. The performance of the CDT cRMP in combination with previously developed commutable calibrators allows for standardization of the currently available commercial measurement procedures for CDT. The cRMP has recently been approved by the IFCC and will be from now on be known as the IFCC-RMP for CDT, while CDT results standardized according to this RMP should be indicated as CDT<sub>IFCC</sub>.</p>},
  author       = {Schellenberg, François and Wielders, Jos and Anton, Raymond and Bianchi, Vincenza and Deenmamode, Jean and Weykamp, Cas and Whitfield, John B and Jeppsson, Jan Olof and Helander, Anders},
  issn         = {0009-8981},
  keyword      = {Alcohol biomarker,CDT,Disialotransferrin,HPLC,Reference Measurement Procedure (RMP),Standardization},
  language     = {eng},
  month        = {02},
  pages        = {91--100},
  publisher    = {Elsevier},
  series       = {Clinica Chimica Acta},
  title        = {IFCC approved HPLC reference measurement procedure for the alcohol consumption biomarker carbohydrate-deficient transferrin (CDT) : Its validation and use},
  url          = {http://dx.doi.org/10.1016/j.cca.2016.12.022},
  volume       = {465},
  year         = {2017},
}