Advanced

Determination of lactose by a novel third generation biosensor based on a cellobiose dehydrogenase and aryl diazonium modified single wall carbon nanotubes electrode

Tasca, Federico LU ; Ludwig, Roland; Gorton, Lo LU and Antiochia, Riccarda (2013) In Sensors and Actuators B: Chemical 177. p.64-69
Abstract
In this paper a new third-generation amperometric biosensor for lactose determination is described. The biosensor is based on the highly efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida (PsCDH) and single walled carbon nanotubes (SWCNT). The SWNCTs were surface modified with aryl diazonium salts of p-phenylenediamine (NH2-PD) and deposited on top of a glassy carbon (GC) electrode. The PsCDH NH2-PD/SWCNT-GC biosensor showed very efficient DET and exhibited an extraordinary high current density of 500 mu M cm(-2) in a 5 mM lactose solution at pH 3.5. The biosensor has a detection limit for lactose of 0.5 mu M, a large linear range from 1 to 150 mu M lactose and a high sensitivity... (More)
In this paper a new third-generation amperometric biosensor for lactose determination is described. The biosensor is based on the highly efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida (PsCDH) and single walled carbon nanotubes (SWCNT). The SWNCTs were surface modified with aryl diazonium salts of p-phenylenediamine (NH2-PD) and deposited on top of a glassy carbon (GC) electrode. The PsCDH NH2-PD/SWCNT-GC biosensor showed very efficient DET and exhibited an extraordinary high current density of 500 mu M cm(-2) in a 5 mM lactose solution at pH 3.5. The biosensor has a detection limit for lactose of 0.5 mu M, a large linear range from 1 to 150 mu M lactose and a high sensitivity (476.8 nA mu M-1 cm(-2)). It shows also a fast response time (4s), good reproducibility (RSD =1.75%) and good stability (half-life 12 days). In addition, it is easy, simple to manufacture, and cheap because a low amount of enzyme is required and highly selective, as no significant interference was observed. For these reasons, it can represent a valid alternative to HPLC measurements for lactose determination in milk and dairy products. (c) 2012 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactose determination, Biosensor, Cellobiose dehydrogenase, Single-walled carbon nanotubes, Aryl diazonium salts
in
Sensors and Actuators B: Chemical
volume
177
pages
64 - 69
publisher
Elsevier
external identifiers
  • wos:000315751000010
  • scopus:84871915846
ISSN
0925-4005
DOI
10.1016/j.snb.2012.10.114
language
English
LU publication?
yes
id
b0282aaa-b1b7-4c17-9823-07c86fc6485b (old id 3657971)
date added to LUP
2013-04-24 12:42:05
date last changed
2019-02-20 06:21:01
@article{b0282aaa-b1b7-4c17-9823-07c86fc6485b,
  abstract     = {In this paper a new third-generation amperometric biosensor for lactose determination is described. The biosensor is based on the highly efficient direct electron transfer (DET) between cellobiose dehydrogenase (CDH) from Phanerochaete sordida (PsCDH) and single walled carbon nanotubes (SWCNT). The SWNCTs were surface modified with aryl diazonium salts of p-phenylenediamine (NH2-PD) and deposited on top of a glassy carbon (GC) electrode. The PsCDH NH2-PD/SWCNT-GC biosensor showed very efficient DET and exhibited an extraordinary high current density of 500 mu M cm(-2) in a 5 mM lactose solution at pH 3.5. The biosensor has a detection limit for lactose of 0.5 mu M, a large linear range from 1 to 150 mu M lactose and a high sensitivity (476.8 nA mu M-1 cm(-2)). It shows also a fast response time (4s), good reproducibility (RSD =1.75%) and good stability (half-life 12 days). In addition, it is easy, simple to manufacture, and cheap because a low amount of enzyme is required and highly selective, as no significant interference was observed. For these reasons, it can represent a valid alternative to HPLC measurements for lactose determination in milk and dairy products. (c) 2012 Elsevier B.V. All rights reserved.},
  author       = {Tasca, Federico and Ludwig, Roland and Gorton, Lo and Antiochia, Riccarda},
  issn         = {0925-4005},
  keyword      = {Lactose determination,Biosensor,Cellobiose dehydrogenase,Single-walled carbon nanotubes,Aryl diazonium salts},
  language     = {eng},
  pages        = {64--69},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Determination of lactose by a novel third generation biosensor based on a cellobiose dehydrogenase and aryl diazonium modified single wall carbon nanotubes electrode},
  url          = {http://dx.doi.org/10.1016/j.snb.2012.10.114},
  volume       = {177},
  year         = {2013},
}