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Methods to Stud MYC-Regulated Cellular Senescence

Tabor, Vedrana ; Bocci, Matteo LU orcid and Larsson, Lars-Gunnar (2013) In Methods in Molecular Biology 1012. p.99-116
Abstract
Studies in primary and tumor cells suggest that MYC plays an important role in regulating cellular senescence, thereby impacting on tumor development. Here we describe different common methods to measure senescence in cell cultures and in tissues. These include measurement of senescence-associated β-galactosidase activity (SA-β-gal), senescence-associated heterochromatin foci (SAHFs), proliferative arrest, morphological changes, and expression and activity of proteins involved in the senescence process, such as p53 and Rb pathway proteins and secretory proteins. It is important to note that there is no unique marker that unambiguously defines a senescent state, and it is therefore necessary to combine measurements of several different... (More)
Studies in primary and tumor cells suggest that MYC plays an important role in regulating cellular senescence, thereby impacting on tumor development. Here we describe different common methods to measure senescence in cell cultures and in tissues. These include measurement of senescence-associated β-galactosidase activity (SA-β-gal), senescence-associated heterochromatin foci (SAHFs), proliferative arrest, morphological changes, and expression and activity of proteins involved in the senescence process, such as p53 and Rb pathway proteins and secretory proteins. It is important to note that there is no unique marker that unambiguously defines a senescent state, and it is therefore necessary to combine measurements of several different markers that together determine whether cells are senescent or not. Measurement of senescence is an important aspect of studies of MYC biology and will improve our understanding of MYC function and regulation both in preclinical and clinical settings. This may form the basis for new concepts of pro-senescence therapy to combat MYC in cancer. (Less)
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author
; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
host publication
The Myc Gene : Methods and Protocols
series title
Methods in Molecular Biology
editor
Soucek, Laura and Sodir, Nicole M.
volume
1012
pages
18 pages
publisher
Springer
external identifiers
  • scopus:84934444574
ISBN
978-1-62703-428-9
978-1-62703-429-6
DOI
10.1007/978-1-62703-429-6_8
language
English
LU publication?
no
id
b0546dda-2b0b-422b-9bc4-0fa9bb0ba635
date added to LUP
2017-10-11 12:11:37
date last changed
2024-01-14 07:13:00
@inbook{b0546dda-2b0b-422b-9bc4-0fa9bb0ba635,
  abstract     = {{Studies in primary and tumor cells suggest that MYC plays an important role in regulating cellular senescence, thereby impacting on tumor development. Here we describe different common methods to measure senescence in cell cultures and in tissues. These include measurement of senescence-associated β-galactosidase activity (SA-β-gal), senescence-associated heterochromatin foci (SAHFs), proliferative arrest, morphological changes, and expression and activity of proteins involved in the senescence process, such as p53 and Rb pathway proteins and secretory proteins. It is important to note that there is no unique marker that unambiguously defines a senescent state, and it is therefore necessary to combine measurements of several different markers that together determine whether cells are senescent or not. Measurement of senescence is an important aspect of studies of MYC biology and will improve our understanding of MYC function and regulation both in preclinical and clinical settings. This may form the basis for new concepts of pro-senescence therapy to combat MYC in cancer.}},
  author       = {{Tabor, Vedrana and Bocci, Matteo and Larsson, Lars-Gunnar}},
  booktitle    = {{The Myc Gene : Methods and Protocols}},
  editor       = {{Soucek, Laura and Sodir, Nicole M.}},
  isbn         = {{978-1-62703-428-9}},
  language     = {{eng}},
  month        = {{08}},
  pages        = {{99--116}},
  publisher    = {{Springer}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Methods to Stud MYC-Regulated Cellular Senescence}},
  url          = {{http://dx.doi.org/10.1007/978-1-62703-429-6_8}},
  doi          = {{10.1007/978-1-62703-429-6_8}},
  volume       = {{1012}},
  year         = {{2013}},
}