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A plant thiolase involved in benzoic acid biosynthesis and volatile benzenoid production

Van Moerkercke, Alex LU ; Schauvinhold, Ines; Pichersky, Eran; Haring, Michel A and Schuurink, Robert C (2009) In Plant Journal 60(2). p.292-302
Abstract

The exact biosynthetic pathways leading to benzoic acid (BA) formation in plants are not known, but labeling experiments indicate the contribution of both beta-oxidative and non-beta-oxidative pathways. In Petunia hybrida BA is a key precursor for the production of volatile benzenoids by its flowers. Using functional genomics, we identified a 3-ketoacyl-CoA thiolase, PhKAT1, which is involved in the benzenoid biosynthetic pathway and the production of BA. PhKAT1 is localised in the peroxisomes, where it is important for the formation of benzoyl-CoA-related compounds. Silencing of PhKAT1 resulted in a major reduction in BA and benzenoid formation, leaving the production of other phenylpropanoid-related volatiles unaffected. During the... (More)

The exact biosynthetic pathways leading to benzoic acid (BA) formation in plants are not known, but labeling experiments indicate the contribution of both beta-oxidative and non-beta-oxidative pathways. In Petunia hybrida BA is a key precursor for the production of volatile benzenoids by its flowers. Using functional genomics, we identified a 3-ketoacyl-CoA thiolase, PhKAT1, which is involved in the benzenoid biosynthetic pathway and the production of BA. PhKAT1 is localised in the peroxisomes, where it is important for the formation of benzoyl-CoA-related compounds. Silencing of PhKAT1 resulted in a major reduction in BA and benzenoid formation, leaving the production of other phenylpropanoid-related volatiles unaffected. During the night, when volatile benzenoid production is highest, it is largely the beta-oxidative pathway that contributes to the formation of BA and benzenoids. Our studies add the benzenoid biosynthetic pathway to the list of pathways in which 3-ketoacyl-CoA thiolases are involved in plants.

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author
publishing date
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Contribution to journal
publication status
published
subject
keywords
Acetyl-CoA C-Acyltransferase, Benzoic Acid, DNA, Complementary, Flowers, Gene Expression Regulation, Plant, Gene Silencing, Molecular Sequence Data, Oils, Volatile, Peroxisomes, Petunia, Plant Proteins, Plants, Genetically Modified, RNA, Plant
in
Plant Journal
volume
60
issue
2
pages
11 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:70349854538
ISSN
1365-313X
DOI
10.1111/j.1365-313X.2009.03953.x
language
English
LU publication?
no
id
b07598d4-af74-4650-886c-7abbcd07b4bd
date added to LUP
2017-11-06 11:32:50
date last changed
2017-12-10 05:13:14
@article{b07598d4-af74-4650-886c-7abbcd07b4bd,
  abstract     = {<p>The exact biosynthetic pathways leading to benzoic acid (BA) formation in plants are not known, but labeling experiments indicate the contribution of both beta-oxidative and non-beta-oxidative pathways. In Petunia hybrida BA is a key precursor for the production of volatile benzenoids by its flowers. Using functional genomics, we identified a 3-ketoacyl-CoA thiolase, PhKAT1, which is involved in the benzenoid biosynthetic pathway and the production of BA. PhKAT1 is localised in the peroxisomes, where it is important for the formation of benzoyl-CoA-related compounds. Silencing of PhKAT1 resulted in a major reduction in BA and benzenoid formation, leaving the production of other phenylpropanoid-related volatiles unaffected. During the night, when volatile benzenoid production is highest, it is largely the beta-oxidative pathway that contributes to the formation of BA and benzenoids. Our studies add the benzenoid biosynthetic pathway to the list of pathways in which 3-ketoacyl-CoA thiolases are involved in plants.</p>},
  author       = {Van Moerkercke, Alex and Schauvinhold, Ines and Pichersky, Eran and Haring, Michel A and Schuurink, Robert C},
  issn         = {1365-313X},
  keyword      = {Acetyl-CoA C-Acyltransferase,Benzoic Acid,DNA, Complementary,Flowers,Gene Expression Regulation, Plant,Gene Silencing,Molecular Sequence Data,Oils, Volatile,Peroxisomes,Petunia,Plant Proteins,Plants, Genetically Modified,RNA, Plant},
  language     = {eng},
  number       = {2},
  pages        = {292--302},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {A plant thiolase involved in benzoic acid biosynthesis and volatile benzenoid production},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2009.03953.x},
  volume       = {60},
  year         = {2009},
}