Characterization of GYP*Mur and novel GYP*Bun-like hybrids in Thai blood donors reveals a qualitatively altered s antigen
(2020) In Vox Sanguinis 115(5). p.472-477- Abstract
Background and objectives: The Mi(a+) GP(B-A-B) hybrid phenotypes occur with a prevalence of 2%–23% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti-s mistyped six S‒s+ RBC units as S‒s‒. Further, alloanti-s was identified in an S+s+ patient. Our objective was to investigate the s antigen further. Materials and methods: DNA from 63 Thai blood donor samples PCR-positive for a GYP(B-A-B) hybrid was sequenced with primers spanning GYPB exons 3–4. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype. Results: DNA sequencing showed that GYP*Mur was carried by 56/63 samples (88·9%)... (More)
Background and objectives: The Mi(a+) GP(B-A-B) hybrid phenotypes occur with a prevalence of 2%–23% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti-s mistyped six S‒s+ RBC units as S‒s‒. Further, alloanti-s was identified in an S+s+ patient. Our objective was to investigate the s antigen further. Materials and methods: DNA from 63 Thai blood donor samples PCR-positive for a GYP(B-A-B) hybrid was sequenced with primers spanning GYPB exons 3–4. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype. Results: DNA sequencing showed that GYP*Mur was carried by 56/63 samples (88·9%) of which 5/56 lacked normal GYPB: three of these were GYP*Mur homozygotes, one was a compound heterozygote carrying GYP*Mur and a GYP*Bun-like allele (designated GYP*Thai), and the fifth sample carried GYP*Mur and another GYP*Bun-like allele. Seven samples (7/63) were GYP*Thai heterozygotes. IgM monoclonal anti-s (P3BER) did not react with the s antigen carried by GP.Mur or GP.Bun, whereas two IgG anti-s showed enhanced reactivity. Conclusions: We confirmed that GYP*Mur is the most frequent variant in Thai blood donors and also identified GYP*Thai with a frequency of 1·1%. We showed that s antigen on Mi(a+) GP(B-A-B) hybrids is qualitatively altered and should be considered when selecting reagents for phenotyping where such hybrids are prevalent, endemically and in blood centres worldwide.
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- author
- Jongruamklang, Philaiphon LU ; Grimsley, Shane ; Thornton, Nicole ; Robb, Janine ; Olsson, Martin L. LU and Storry, Jill R. LU
- organization
- publishing date
- 2020-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- blood group antigen, glycophorin, GP.Mur
- in
- Vox Sanguinis
- volume
- 115
- issue
- 5
- pages
- 6 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:32201961
- scopus:85082142107
- ISSN
- 0042-9007
- DOI
- 10.1111/vox.12909
- project
- Blood group polymorphism and its relationship to malaria susceptibility
- language
- English
- LU publication?
- yes
- id
- b0bebad4-5044-4979-8b29-4718e0bee9c9
- date added to LUP
- 2020-04-08 14:47:45
- date last changed
- 2024-08-21 18:56:44
@article{b0bebad4-5044-4979-8b29-4718e0bee9c9, abstract = {{<p>Background and objectives: The Mi(a+) GP(B-A-B) hybrid phenotypes occur with a prevalence of 2%–23% across Southeast Asia. While the s antigen is alleged to be altered, no evidence for specific variants is known. Screening using a monoclonal IgM anti-s mistyped six S‒s+ RBC units as S‒s‒. Further, alloanti-s was identified in an S+s+ patient. Our objective was to investigate the s antigen further. Materials and methods: DNA from 63 Thai blood donor samples PCR-positive for a GYP(B-A-B) hybrid was sequenced with primers spanning GYPB exons 3–4. Flow cytometry was used for semiquantitative analysis of s expression and correlated with the glycophorin genotype. Results: DNA sequencing showed that GYP*Mur was carried by 56/63 samples (88·9%) of which 5/56 lacked normal GYPB: three of these were GYP*Mur homozygotes, one was a compound heterozygote carrying GYP*Mur and a GYP*Bun-like allele (designated GYP*Thai), and the fifth sample carried GYP*Mur and another GYP*Bun-like allele. Seven samples (7/63) were GYP*Thai heterozygotes. IgM monoclonal anti-s (P3BER) did not react with the s antigen carried by GP.Mur or GP.Bun, whereas two IgG anti-s showed enhanced reactivity. Conclusions: We confirmed that GYP*Mur is the most frequent variant in Thai blood donors and also identified GYP*Thai with a frequency of 1·1%. We showed that s antigen on Mi(a+) GP(B-A-B) hybrids is qualitatively altered and should be considered when selecting reagents for phenotyping where such hybrids are prevalent, endemically and in blood centres worldwide.</p>}}, author = {{Jongruamklang, Philaiphon and Grimsley, Shane and Thornton, Nicole and Robb, Janine and Olsson, Martin L. and Storry, Jill R.}}, issn = {{0042-9007}}, keywords = {{blood group antigen; glycophorin; GP.Mur}}, language = {{eng}}, number = {{5}}, pages = {{472--477}}, publisher = {{Wiley-Blackwell}}, series = {{Vox Sanguinis}}, title = {{Characterization of GYP*Mur and novel GYP*Bun-like hybrids in Thai blood donors reveals a qualitatively altered s antigen}}, url = {{http://dx.doi.org/10.1111/vox.12909}}, doi = {{10.1111/vox.12909}}, volume = {{115}}, year = {{2020}}, }