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Evaluation and modification of lanthanum-based flocculation for isolation of bacteria from water samples

Jansson, Linda LU ; Eriksson, Ronnie; Hedman, Johannes LU and Lavander, Moa (2018) In Biotechnology Reports 19.
Abstract

Molecular detection of pathogenic microorganisms in drinking and natural water is often challenged by low concentrations of the sought-after agents. Convenient methods to concentrate bacteria from water samples ranging from 1-10 L are highly warranted. Here we account for the evaluation of a lanthanum-based flocculation method to concentrate bacteria from water samples, applying four different bacterial species in tap water as well as river water. Our results show that the success of lanthanum-based flocculation is determined by both the bacterial species and the nature of the water sample. For tap water, satisfying flocculation efficiencies (above 60 %) were only reached for autoclaved water samples. However, the performance of the... (More)

Molecular detection of pathogenic microorganisms in drinking and natural water is often challenged by low concentrations of the sought-after agents. Convenient methods to concentrate bacteria from water samples ranging from 1-10 L are highly warranted. Here we account for the evaluation of a lanthanum-based flocculation method to concentrate bacteria from water samples, applying four different bacterial species in tap water as well as river water. Our results show that the success of lanthanum-based flocculation is determined by both the bacterial species and the nature of the water sample. For tap water, satisfying flocculation efficiencies (above 60 %) were only reached for autoclaved water samples. However, the performance of the lanthanum-based flocculation method for non-autoclaved water was markedly improved by the addition of 20 mM bicarbonate to increase alkalinity. Our modified flocculation protocol may be applied as an alternative concentration method for bacteria in water samples of one liter or more.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Reports
volume
19
publisher
Elsevier
external identifiers
  • scopus:85048775086
ISSN
2215-017X
DOI
10.1016/j.btre.2018.e00267
language
English
LU publication?
yes
id
b0e263a2-0481-4098-93f8-6d2090c99c2b
date added to LUP
2018-07-04 10:25:37
date last changed
2019-02-20 11:21:31
@article{b0e263a2-0481-4098-93f8-6d2090c99c2b,
  abstract     = {<p>Molecular detection of pathogenic microorganisms in drinking and natural water is often challenged by low concentrations of the sought-after agents. Convenient methods to concentrate bacteria from water samples ranging from 1-10 L are highly warranted. Here we account for the evaluation of a lanthanum-based flocculation method to concentrate bacteria from water samples, applying four different bacterial species in tap water as well as river water. Our results show that the success of lanthanum-based flocculation is determined by both the bacterial species and the nature of the water sample. For tap water, satisfying flocculation efficiencies (above 60 %) were only reached for autoclaved water samples. However, the performance of the lanthanum-based flocculation method for non-autoclaved water was markedly improved by the addition of 20 mM bicarbonate to increase alkalinity. Our modified flocculation protocol may be applied as an alternative concentration method for bacteria in water samples of one liter or more.</p>},
  articleno    = {e00267},
  author       = {Jansson, Linda and Eriksson, Ronnie and Hedman, Johannes and Lavander, Moa},
  issn         = {2215-017X},
  language     = {eng},
  month        = {09},
  publisher    = {Elsevier},
  series       = {Biotechnology Reports},
  title        = {Evaluation and modification of lanthanum-based flocculation for isolation of bacteria from water samples},
  url          = {http://dx.doi.org/10.1016/j.btre.2018.e00267},
  volume       = {19},
  year         = {2018},
}