NMR relaxation times of trabecular bone - reproducibility, relationships to tissue structure and effects of sample freezing
(2010) In Physics in Medicine and Biology 55(23). p.7363-7555- Abstract
- Abstract in Undetermined
Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy before and... (More) - Abstract in Undetermined
Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy before and after freezing. The reproducibility of NMR relaxation parameters, as expressed by the coefficient of variation, ranged from 3.1% to 27.9%. In fresh samples, some correlations between NMR and structural parameters (Tb.N, Tb.Sp) were significant (e.g. the relaxation rate for T(2) of fat versus Tb.Sp: r = -0.716, p < 0.01). Freezing did not significantly change the NMR relaxation times but the correlations between relaxation parameters and the μCT structural parameters were not statistically significant after freezing, suggesting some nonsystematic alterations of the marrow structure. Therefore, the use of frozen bone samples for NMR relaxation studies may provide inferior information about the trabecular bone structure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2277238
- author
- Pranter, Viktoria ; Isaksson, Hanna LU ; Närväinen, Johanna ; Lammentausta, Eveliina ; Nissi, Mikko ; Avela, Janne ; Gröhn, Olli and Jurvelin, Jukka
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physics in Medicine and Biology
- volume
- 55
- issue
- 23
- pages
- 7363 - 7555
- publisher
- IOP Publishing
- external identifiers
-
- scopus:78650064279
- ISSN
- 1361-6560
- DOI
- 10.1088/0031-9155/55/23/012
- language
- English
- LU publication?
- no
- id
- b12ac467-cf62-463e-bfd1-552402217246 (old id 2277238)
- date added to LUP
- 2016-04-04 07:33:20
- date last changed
- 2023-09-05 12:19:08
@article{b12ac467-cf62-463e-bfd1-552402217246, abstract = {{Abstract in Undetermined<br> Nuclear magnetic resonance (NMR) spectroscopy provides a potential tool for non-invasive evaluation of the trabecular bone structure. The objective of this study was to determine the reproducibility of the NMR relaxation parameters (T(2), Carr-Purcel-T(2), T(1ρ)) for fat and water and relate those to the structural parameters obtained by micro-computed tomography (μCT). Especially, we aimed to evaluate the effect of freezing on the relaxation parameters. For storing bone samples, freezing is the standard procedure during which the biochemical and cellular organization of the bone marrow may be affected. Bovine trabecular bone samples were stored at -20 °C for 7 days and measured by NMR spectroscopy before and after freezing. The reproducibility of NMR relaxation parameters, as expressed by the coefficient of variation, ranged from 3.1% to 27.9%. In fresh samples, some correlations between NMR and structural parameters (Tb.N, Tb.Sp) were significant (e.g. the relaxation rate for T(2) of fat versus Tb.Sp: r = -0.716, p < 0.01). Freezing did not significantly change the NMR relaxation times but the correlations between relaxation parameters and the μCT structural parameters were not statistically significant after freezing, suggesting some nonsystematic alterations of the marrow structure. Therefore, the use of frozen bone samples for NMR relaxation studies may provide inferior information about the trabecular bone structure.}}, author = {{Pranter, Viktoria and Isaksson, Hanna and Närväinen, Johanna and Lammentausta, Eveliina and Nissi, Mikko and Avela, Janne and Gröhn, Olli and Jurvelin, Jukka}}, issn = {{1361-6560}}, language = {{eng}}, number = {{23}}, pages = {{7363--7555}}, publisher = {{IOP Publishing}}, series = {{Physics in Medicine and Biology}}, title = {{NMR relaxation times of trabecular bone - reproducibility, relationships to tissue structure and effects of sample freezing}}, url = {{http://dx.doi.org/10.1088/0031-9155/55/23/012}}, doi = {{10.1088/0031-9155/55/23/012}}, volume = {{55}}, year = {{2010}}, }