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Characterization of the predominant basic protein in human seminal plasma, one cleavage product of the major seminal vesicle protein

Lilja, H. LU orcid ; Laurell, C. B. LU and Jeppsson, J. O. LU (1984) In Scandinavian Journal of Clinical and Laboratory Investigation 44(5). p.439-446
Abstract

From liquefied human seminal plasma, we purified the predominant basic protein which appears following liquefaction of coagulated semen. The protein was purified in the presence of di-isopropylfluorophosphate to retard its degradation. Heparin-Sepharose® chromatography was followed by gel filtration (Biogel® P 60) and by fast performance liquid chromatography on a reversed phase column (C8). The basic protein is a single polypeptide chain with an apparent molecular mass of 12.8 kDa, and has a PI value between that of trypsinogen (9.3) and cytochrome C (10.3). The protein contains no carbohydrate, is rich in histidine, glutamate, and lysine, but is devoid of both cysteine and methionine. The amino-terminal portion of the... (More)

From liquefied human seminal plasma, we purified the predominant basic protein which appears following liquefaction of coagulated semen. The protein was purified in the presence of di-isopropylfluorophosphate to retard its degradation. Heparin-Sepharose® chromatography was followed by gel filtration (Biogel® P 60) and by fast performance liquid chromatography on a reversed phase column (C8). The basic protein is a single polypeptide chain with an apparent molecular mass of 12.8 kDa, and has a PI value between that of trypsinogen (9.3) and cytochrome C (10.3). The protein contains no carbohydrate, is rich in histidine, glutamate, and lysine, but is devoid of both cysteine and methionine. The amino-terminal portion of the protein sequence is unique: HNKQEGRDHDKSKG HFHRVVIHHKGGKAHRG-.A specific rabbit antiserum was raised against the 12.8 kDa basic protein. The protein was found to be unique to seminal plasma among all extracellular fluids examined. Three immunologically related 52 kDa, 71 kDa, and 76 kDa proteins were identified in seminal vesicle secretion when it had been reduced. Prostatic enzyme(s) degraded these proteins to the 12.8 kDa basic protein and several other basic proteins with apparent molecular masses below 18 kDa.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Peptide fragments, Prostate, Protease inhibitors, Semen, Seminal vesicles
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
44
issue
5
pages
439 - 446
publisher
Informa Healthcare
external identifiers
  • pmid:6484484
  • scopus:0021233719
ISSN
0036-5513
DOI
10.3109/00365518409083835
language
English
LU publication?
yes
id
b12b7852-9d44-456f-a434-f512b39ecd53
date added to LUP
2022-12-06 16:58:54
date last changed
2024-01-03 19:32:27
@article{b12b7852-9d44-456f-a434-f512b39ecd53,
  abstract     = {{<p>From liquefied human seminal plasma, we purified the predominant basic protein which appears following liquefaction of coagulated semen. The protein was purified in the presence of di-isopropylfluorophosphate to retard its degradation. Heparin-Sepharose® chromatography was followed by gel filtration (Biogel® P 60) and by fast performance liquid chromatography on a reversed phase column (C<sub>8</sub>). The basic protein is a single polypeptide chain with an apparent molecular mass of 12.8 kDa, and has a PI value between that of trypsinogen (9.3) and cytochrome C (10.3). The protein contains no carbohydrate, is rich in histidine, glutamate, and lysine, but is devoid of both cysteine and methionine. The amino-terminal portion of the protein sequence is unique: HNKQEGRDHDKSKG HFHRVVIHHKGGKAHRG-.A specific rabbit antiserum was raised against the 12.8 kDa basic protein. The protein was found to be unique to seminal plasma among all extracellular fluids examined. Three immunologically related 52 kDa, 71 kDa, and 76 kDa proteins were identified in seminal vesicle secretion when it had been reduced. Prostatic enzyme(s) degraded these proteins to the 12.8 kDa basic protein and several other basic proteins with apparent molecular masses below 18 kDa.</p>}},
  author       = {{Lilja, H. and Laurell, C. B. and Jeppsson, J. O.}},
  issn         = {{0036-5513}},
  keywords     = {{Peptide fragments; Prostate; Protease inhibitors; Semen; Seminal vesicles}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{439--446}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Characterization of the predominant basic protein in human seminal plasma, one cleavage product of the major seminal vesicle protein}},
  url          = {{http://dx.doi.org/10.3109/00365518409083835}},
  doi          = {{10.3109/00365518409083835}},
  volume       = {{44}},
  year         = {{1984}},
}