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Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers

Luchini, Alessandra ; Delhom, Robin LU ; Cristiglio, Viviana ; Knecht, Wolfgang LU ; Wacklin-Knecht, Hanna LU and Fragneto, Giovanna (2020) In Chemistry and Physics of Lipids 227.
Abstract

Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids... (More)

Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids extracted from the yeast Pichia pastoris. The data show that the effect of ergosterol on membranes prepared from the natural phospholipid extract rich in unsaturated acyl chains, differs from what has been observed previously in membranes rich in saturated phospholipids. In contrast to cholesterol in synthetic phospholipid membranes, the presence of ergosterol up to 30 mol % in yeast phospholipid membranes only slightly altered the multilayer structure. In particular, only a small decrease in the multilayer d-spacing was observed as function of increasing ergosterol concentrations. This result highlights the need for further investigation to elucidate the effects of ergosterol in biological lipid mixtures.

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organization
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Contribution to journal
publication status
published
subject
keywords
Deuterated natural phospholipids, Ergosterol, Lipid multilayers, Neutron diffraction
in
Chemistry and Physics of Lipids
volume
227
article number
104873
publisher
Elsevier
external identifiers
  • pmid:31926858
  • scopus:85077928019
ISSN
0009-3084
DOI
10.1016/j.chemphyslip.2020.104873
language
English
LU publication?
yes
id
b15d08d6-3ca6-438b-b3be-24bb40793a3f
date added to LUP
2020-01-30 16:25:30
date last changed
2020-07-08 05:11:37
@article{b15d08d6-3ca6-438b-b3be-24bb40793a3f,
  abstract     = {<p>Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids extracted from the yeast Pichia pastoris. The data show that the effect of ergosterol on membranes prepared from the natural phospholipid extract rich in unsaturated acyl chains, differs from what has been observed previously in membranes rich in saturated phospholipids. In contrast to cholesterol in synthetic phospholipid membranes, the presence of ergosterol up to 30 mol % in yeast phospholipid membranes only slightly altered the multilayer structure. In particular, only a small decrease in the multilayer d-spacing was observed as function of increasing ergosterol concentrations. This result highlights the need for further investigation to elucidate the effects of ergosterol in biological lipid mixtures.</p>},
  author       = {Luchini, Alessandra and Delhom, Robin and Cristiglio, Viviana and Knecht, Wolfgang and Wacklin-Knecht, Hanna and Fragneto, Giovanna},
  issn         = {0009-3084},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Chemistry and Physics of Lipids},
  title        = {Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers},
  url          = {http://dx.doi.org/10.1016/j.chemphyslip.2020.104873},
  doi          = {10.1016/j.chemphyslip.2020.104873},
  volume       = {227},
  year         = {2020},
}