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Designing an Autonomous Integrated Downstream Sequence From a Batch Separation Process − An Industrial Case Study

Löfgren, Anton LU ; Andersson, Niklas LU ; Sellberg, Anton LU ; Nilsson, Bernt LU ; Löfgren, Magnus and Wood, Susanne (2018) In Biotechnology Journal
Abstract
This work is a proof of concept of how a sequence of industrial batch separation steps together are used to form an integrated autonomous downstream process. The sequence in this case study consisted of an anion chromatography step, virus inactivation and finally a hydrophobic chromatography step. Moving from batch to integrated separation minimizes hold‐up times, storage tanks, and required equipment. The conversion from batch to integrated mode is achieved by extracting operating points and separation data from batch chromatograms. The integrated separation process is realized on an ÄKTA Pure controlled by an open research software called Orbit, making it possible to operate complex process configurations including multiple steps. The... (More)
This work is a proof of concept of how a sequence of industrial batch separation steps together are used to form an integrated autonomous downstream process. The sequence in this case study consisted of an anion chromatography step, virus inactivation and finally a hydrophobic chromatography step. Moving from batch to integrated separation minimizes hold‐up times, storage tanks, and required equipment. The conversion from batch to integrated mode is achieved by extracting operating points and separation data from batch chromatograms. The integrated separation process is realized on an ÄKTA Pure controlled by an open research software called Orbit, making it possible to operate complex process configurations including multiple steps. The results from this case study is the principle and method of the steps taken to automation, achieving a more continuous and efficient downstream process. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Journal
pages
5 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:85041107635
ISSN
1860-6768
DOI
10.1002/biot.201700691
language
English
LU publication?
yes
id
b1b19748-56de-4d77-a660-9067b115ef94
date added to LUP
2018-04-10 10:38:38
date last changed
2018-04-15 04:51:44
@article{b1b19748-56de-4d77-a660-9067b115ef94,
  abstract     = {This work is a proof of concept of how a sequence of industrial batch separation steps together are used to form an integrated autonomous downstream process. The sequence in this case study consisted of an anion chromatography step, virus inactivation and finally a hydrophobic chromatography step. Moving from batch to integrated separation minimizes hold‐up times, storage tanks, and required equipment. The conversion from batch to integrated mode is achieved by extracting operating points and separation data from batch chromatograms. The integrated separation process is realized on an ÄKTA Pure controlled by an open research software called Orbit, making it possible to operate complex process configurations including multiple steps. The results from this case study is the principle and method of the steps taken to automation, achieving a more continuous and efficient downstream process. },
  author       = {Löfgren, Anton and Andersson, Niklas and Sellberg, Anton and Nilsson, Bernt and Löfgren, Magnus and Wood, Susanne},
  issn         = {1860-6768},
  language     = {eng},
  month        = {01},
  pages        = {5},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology Journal},
  title        = {Designing an Autonomous Integrated Downstream Sequence From a Batch Separation Process − An Industrial Case Study},
  url          = {http://dx.doi.org/10.1002/biot.201700691},
  year         = {2018},
}