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Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand.

Fisher, Zoe LU ; von Schantz, Laura LU ; Håkansson, Maria LU ; Logan, Derek LU and Ohlin, Mats LU (2015) In Biochemistry 54(42). p.6435-6438
Abstract
Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM-ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM-ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
54
issue
42
pages
6435 - 6438
publisher
The American Chemical Society
external identifiers
  • pmid:26451738
  • wos:000363916200001
  • scopus:84945567452
ISSN
0006-2960
DOI
10.1021/acs.biochem.5b01058
language
English
LU publication?
yes
id
b21ac34a-acc3-4ec1-a053-fdfcaa73cf4d (old id 8155614)
date added to LUP
2015-11-15 22:54:06
date last changed
2019-01-06 04:19:56
@article{b21ac34a-acc3-4ec1-a053-fdfcaa73cf4d,
  abstract     = {Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM-ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM-ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.},
  author       = {Fisher, Zoe and von Schantz, Laura and Håkansson, Maria and Logan, Derek and Ohlin, Mats},
  issn         = {0006-2960},
  language     = {eng},
  number       = {42},
  pages        = {6435--6438},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand.},
  url          = {http://dx.doi.org/10.1021/acs.biochem.5b01058},
  volume       = {54},
  year         = {2015},
}