Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand.
(2015) In Biochemistry 54(42). p.6435-6438- Abstract
- Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM-ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM-ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/record/8155614
- author
- Fisher, Zoe LU ; von Schantz, Laura LU ; Håkansson, Maria LU ; Logan, Derek LU and Ohlin, Mats LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemistry
- volume
- 54
- issue
- 42
- pages
- 6435 - 6438
- publisher
- The American Chemical Society
- external identifiers
-
- pmid:26451738
- wos:000363916200001
- scopus:84945567452
- ISSN
- 0006-2960
- DOI
- 10.1021/acs.biochem.5b01058
- language
- English
- LU publication?
- yes
- id
- b21ac34a-acc3-4ec1-a053-fdfcaa73cf4d (old id 8155614)
- date added to LUP
- 2015-11-15 22:54:06
- date last changed
- 2019-01-06 04:19:56
@article{b21ac34a-acc3-4ec1-a053-fdfcaa73cf4d, abstract = {Carbohydrate-binding modules (CBMs) are key components of many carbohydrate-modifying enzymes. CBMs affect the activity of these enzymes by modulating bonding and catalysis. To further characterize and study CBM-ligand binding interactions, neutron crystallographic studies of an engineered family 4-type CBM in complex with a branched xyloglucan ligand were conducted. The first neutron crystal structure of a CBM-ligand complex reported here shows numerous atomic details of hydrogen bonding and water-mediated interactions and reveals the charged state of key binding cleft amino acid side chains.}, author = {Fisher, Zoe and von Schantz, Laura and Håkansson, Maria and Logan, Derek and Ohlin, Mats}, issn = {0006-2960}, language = {eng}, number = {42}, pages = {6435--6438}, publisher = {The American Chemical Society}, series = {Biochemistry}, title = {Neutron Crystallographic Studies Reveal Hydrogen Bond and Water-Mediated Interactions between a Carbohydrate-Binding Module and Its Bound Carbohydrate Ligand.}, url = {http://dx.doi.org/10.1021/acs.biochem.5b01058}, volume = {54}, year = {2015}, }