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Time between anterior cruciate ligament injury and reconstruction and cartilage metabolism six-months following reconstruction

Davis, Hope C. ; Spang, Jeffery T. ; Loeser, Richard F. ; Larsson, Staffan LU ; Ulici, Veronica ; Troy Blackburn, J. ; Creighton, R. Alexander ; Kamath, Ganesh M. ; Jordan, Joanne M. and Marshall, Stephen W. , et al. (2018) In Knee 25(2). p.296-305
Abstract

Background: To determine the association between time from injury to ACL reconstruction (TimeInjury-ACLR) and biochemical markers of cartilage metabolism and inflammation six months following ACL reconstruction (ACLR). Methods: Individuals with a unilateral ACL injury were enrolled at initial presentation in the orthopedic clinic; blood was collected six months following ACLR. Enzyme-linked immunosorbent assays were used to analyze the ratio of serum concentrations of type-II collagen breakdown (C2C) to synthesis (CPII), plasma matrix metalloproteinase-3 (MMP-3), interleukin-6 (IL-6), and serum aggrecan neoepitope (ARGS). We used separate linear regressions to assess associations between biochemical markers and... (More)

Background: To determine the association between time from injury to ACL reconstruction (TimeInjury-ACLR) and biochemical markers of cartilage metabolism and inflammation six months following ACL reconstruction (ACLR). Methods: Individuals with a unilateral ACL injury were enrolled at initial presentation in the orthopedic clinic; blood was collected six months following ACLR. Enzyme-linked immunosorbent assays were used to analyze the ratio of serum concentrations of type-II collagen breakdown (C2C) to synthesis (CPII), plasma matrix metalloproteinase-3 (MMP-3), interleukin-6 (IL-6), and serum aggrecan neoepitope (ARGS). We used separate linear regressions to assess associations between biochemical markers and TimeInjury-ACLR. Results: Twenty-two participants (50% females, mean [SD], age 21.9 [4.5] years old; BMI 23.8 [2.6] kg/m2) completed the study. TimeInjury-ACLR ranged from nine to 67days (31.0 [14.4days]). Greater TimeInjury-ACLR predicted greater serum C2C:CPII ratios six months following ACLR (C2C:CPII=0.15 [0.02], R2 =0.213, P=0.030). Males (R2 =0.733, P=0.001) but not females (R2 =0.030, P=0.609) demonstrated a significant association between greater C2C:CPII and TimeInjury-ACLR at the six-month follow-up exam. TimeInjury-ACLR did not associate with IL-6, MMP-3, or ARGS at six months. Conclusions: Greater time between injury and ACL reconstruction was associated with greater serum C2C:CPII six months following ACLR in males but not females, and IL-6, MMP-3, and ARGS levels were not associated with TimeInjury-ACLR in males or females. The time between ACL injury and ACLR may affect collagen metabolism in males and should be further investigated in a larger study along with other patient-relevant outcomes.

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Contribution to journal
publication status
published
subject
keywords
ACLR, Biomarkers, C-propeptide of type II procollagen, C2C, CPII, Type-II collagen cleavage product
in
Knee
volume
25
issue
2
pages
296 - 305
publisher
Elsevier
external identifiers
  • scopus:85042940337
  • pmid:29525545
ISSN
0968-0160
DOI
10.1016/j.knee.2018.01.009
language
English
LU publication?
yes
id
b2d621e4-9b0a-4762-bc8c-c13f9a679307
date added to LUP
2018-03-23 10:06:57
date last changed
2020-01-16 03:17:24
@article{b2d621e4-9b0a-4762-bc8c-c13f9a679307,
  abstract     = {<p>Background: To determine the association between time from injury to ACL reconstruction (Time<sup>Injury-ACLR</sup>) and biochemical markers of cartilage metabolism and inflammation six months following ACL reconstruction (ACLR). Methods: Individuals with a unilateral ACL injury were enrolled at initial presentation in the orthopedic clinic; blood was collected six months following ACLR. Enzyme-linked immunosorbent assays were used to analyze the ratio of serum concentrations of type-II collagen breakdown (C2C) to synthesis (CPII), plasma matrix metalloproteinase-3 (MMP-3), interleukin-6 (IL-6), and serum aggrecan neoepitope (ARGS). We used separate linear regressions to assess associations between biochemical markers and Time<sup>Injury-ACLR</sup>. Results: Twenty-two participants (50% females, mean [SD], age 21.9 [4.5] years old; BMI 23.8 [2.6] kg/m<sup>2</sup>) completed the study. Time<sup>Injury-ACLR</sup> ranged from nine to 67days (31.0 [14.4days]). Greater Time<sup>Injury-ACLR</sup> predicted greater serum C2C:CPII ratios six months following ACLR (C2C:CPII=0.15 [0.02], R<sup>2</sup> =0.213, P=0.030). Males (R<sup>2</sup> =0.733, P=0.001) but not females (R<sup>2</sup> =0.030, P=0.609) demonstrated a significant association between greater C2C:CPII and Time<sup>Injury-ACLR</sup> at the six-month follow-up exam. Time<sup>Injury-ACLR</sup> did not associate with IL-6, MMP-3, or ARGS at six months. Conclusions: Greater time between injury and ACL reconstruction was associated with greater serum C2C:CPII six months following ACLR in males but not females, and IL-6, MMP-3, and ARGS levels were not associated with Time<sup>Injury-ACLR</sup> in males or females. The time between ACL injury and ACLR may affect collagen metabolism in males and should be further investigated in a larger study along with other patient-relevant outcomes.</p>},
  author       = {Davis, Hope C. and Spang, Jeffery T. and Loeser, Richard F. and Larsson, Staffan and Ulici, Veronica and Troy Blackburn, J. and Creighton, R. Alexander and Kamath, Ganesh M. and Jordan, Joanne M. and Marshall, Stephen W. and Pietrosimone, Brian},
  issn         = {0968-0160},
  language     = {eng},
  month        = {03},
  number       = {2},
  pages        = {296--305},
  publisher    = {Elsevier},
  series       = {Knee},
  title        = {Time between anterior cruciate ligament injury and reconstruction and cartilage metabolism six-months following reconstruction},
  url          = {http://dx.doi.org/10.1016/j.knee.2018.01.009},
  doi          = {10.1016/j.knee.2018.01.009},
  volume       = {25},
  year         = {2018},
}