Human monocytes and neutrophils store transforming growth factor-α in a subpopulation of cytoplasmic granules
(1997) In Blood 90(3). p.1255-1266- Abstract
Transforming growth factor-α (TGF-α) exerts several effects on target cells, such as neovascularization promotion and mitogenic signalling. Using immunoelectron microscopy, we show that monocytes and neutrophils, store TGF- α in cytoplasmic granules. In monocytes, TGF-α did not colocalize with components of peroxidase-positive granules or with albumin of secretory vesicles. Furthermore, no colocalization of TGF-α with components of azurophilic or specific granules or secretory vesicles was observed in neutrophils. Activated monocytes and tissue-macrophages contained much less TGF-α-positive granules, suggesting TGF-α release. Western blot analysis showed a protein of 10 kD inlysates of monocytes. TGF-α mRNA was detected in monocytoid... (More)
Transforming growth factor-α (TGF-α) exerts several effects on target cells, such as neovascularization promotion and mitogenic signalling. Using immunoelectron microscopy, we show that monocytes and neutrophils, store TGF- α in cytoplasmic granules. In monocytes, TGF-α did not colocalize with components of peroxidase-positive granules or with albumin of secretory vesicles. Furthermore, no colocalization of TGF-α with components of azurophilic or specific granules or secretory vesicles was observed in neutrophils. Activated monocytes and tissue-macrophages contained much less TGF-α-positive granules, suggesting TGF-α release. Western blot analysis showed a protein of 10 kD inlysates of monocytes. TGF-α mRNA was detected in monocytoid cells from the bone marrow by in situ hybridization. This study shows for the first time that monocytes and neutrophils contain TGF-α in all stages of maturation and that TGF-α in monocytes is stored in a large population of peroxidase-negative granules suggesting a function for these granules. Monocytes and neutrophils are important effector cells in inflammatory reactions. The present finding that these cells contain TGF-α might explain complications such as fibrosis and neoplastic transformation, caused by chronic inflammation.
(Less)
- author
- Calafat, Jero ; Janssen, Hans ; Ståhle-Bäckdahl, Mona ; Zuurbier, Astrid E M ; Knol, Edward F. and Egesten, Arne LU
- publishing date
- 1997-08-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 90
- issue
- 3
- pages
- 12 pages
- publisher
- American Society of Hematology
- external identifiers
-
- scopus:0030848105
- pmid:9242560
- ISSN
- 0006-4971
- DOI
- 10.1182/blood.V90.3.1255.1255_1255_1266
- language
- English
- LU publication?
- no
- id
- b2dfa8c5-7470-4659-8c5e-79a2c3518eb5
- date added to LUP
- 2019-06-19 13:09:27
- date last changed
- 2024-01-30 22:55:06
@article{b2dfa8c5-7470-4659-8c5e-79a2c3518eb5,
abstract = {{<p>Transforming growth factor-α (TGF-α) exerts several effects on target cells, such as neovascularization promotion and mitogenic signalling. Using immunoelectron microscopy, we show that monocytes and neutrophils, store TGF- α in cytoplasmic granules. In monocytes, TGF-α did not colocalize with components of peroxidase-positive granules or with albumin of secretory vesicles. Furthermore, no colocalization of TGF-α with components of azurophilic or specific granules or secretory vesicles was observed in neutrophils. Activated monocytes and tissue-macrophages contained much less TGF-α-positive granules, suggesting TGF-α release. Western blot analysis showed a protein of 10 kD inlysates of monocytes. TGF-α mRNA was detected in monocytoid cells from the bone marrow by in situ hybridization. This study shows for the first time that monocytes and neutrophils contain TGF-α in all stages of maturation and that TGF-α in monocytes is stored in a large population of peroxidase-negative granules suggesting a function for these granules. Monocytes and neutrophils are important effector cells in inflammatory reactions. The present finding that these cells contain TGF-α might explain complications such as fibrosis and neoplastic transformation, caused by chronic inflammation.</p>}},
author = {{Calafat, Jero and Janssen, Hans and Ståhle-Bäckdahl, Mona and Zuurbier, Astrid E M and Knol, Edward F. and Egesten, Arne}},
issn = {{0006-4971}},
language = {{eng}},
month = {{08}},
number = {{3}},
pages = {{1255--1266}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{Human monocytes and neutrophils store transforming growth factor-α in a subpopulation of cytoplasmic granules}},
url = {{http://dx.doi.org/10.1182/blood.V90.3.1255.1255_1255_1266}},
doi = {{10.1182/blood.V90.3.1255.1255_1255_1266}},
volume = {{90}},
year = {{1997}},
}