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Protein-based strategies to identify and isolate bacterial virulence factors

Lood, Rolf LU and Frick, Inga Maria LU (2017) In Methods in Molecular Biology 1535. p.3-15
Abstract

Protein–protein interactions play important roles in bacterial pathogenesis. Surface-bound or secreted bacterial proteins are key in mediating bacterial virulence. Thus, these factors are of high importance to study in order to elucidate the molecular mechanisms behind bacterial pathogenesis. Here, we present a protein-based strategy that can be used to identify and isolate bacterial proteins of importance for bacterial virulence, and allow for identification of both unknown host and bacterial factors. The methods described have among others successfully been used to identify and characterize several IgG-binding proteins, including protein G, protein H, and protein L.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Affinity purification, Bacteria, Plasma adsorption, Release of bacterial surface proteins, Virulence factors
in
Methods in Molecular Biology
volume
1535
pages
13 pages
publisher
Humana Press
external identifiers
  • scopus:85005949218
ISSN
10643745
DOI
10.1007/978-1-4939-6673-8_1
language
English
LU publication?
yes
id
b3172a03-c2fe-492f-ad33-3d642e2e9dc7
date added to LUP
2017-03-17 09:43:20
date last changed
2018-01-07 11:56:10
@inbook{b3172a03-c2fe-492f-ad33-3d642e2e9dc7,
  abstract     = {<p>Protein–protein interactions play important roles in bacterial pathogenesis. Surface-bound or secreted bacterial proteins are key in mediating bacterial virulence. Thus, these factors are of high importance to study in order to elucidate the molecular mechanisms behind bacterial pathogenesis. Here, we present a protein-based strategy that can be used to identify and isolate bacterial proteins of importance for bacterial virulence, and allow for identification of both unknown host and bacterial factors. The methods described have among others successfully been used to identify and characterize several IgG-binding proteins, including protein G, protein H, and protein L.</p>},
  author       = {Lood, Rolf and Frick, Inga Maria},
  issn         = {10643745},
  keyword      = {Affinity purification,Bacteria,Plasma adsorption,Release of bacterial surface proteins,Virulence factors},
  language     = {eng},
  pages        = {3--15},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Protein-based strategies to identify and isolate bacterial virulence factors},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6673-8_1},
  volume       = {1535},
  year         = {2017},
}