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An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Krishnan, Rajaram; Charlot, David; Kumosa, Lucas LU ; Hanna, William; Lu, Jerry; Sonnenberg, Avery and Heller, Michael (2011) 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 In Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE p.373-376
Abstract

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Please use this url to cite or link to this publication:
author
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
pages
373 - 376
conference name
2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
external identifiers
  • scopus:84862951501
ISBN
9781457714696
DOI
10.1109/BioCAS.2011.6107805
language
English
LU publication?
no
id
b33c3e7e-65bc-4b5e-9c81-f229b44837ea
date added to LUP
2017-06-23 10:49:22
date last changed
2017-11-16 15:53:42
@inproceedings{b33c3e7e-65bc-4b5e-9c81-f229b44837ea,
  abstract     = {<p>The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at &gt;500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.</p>},
  author       = {Krishnan, Rajaram and Charlot, David and Kumosa, Lucas and Hanna, William and Lu, Jerry and Sonnenberg, Avery and Heller, Michael},
  booktitle    = {Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE},
  isbn         = {9781457714696},
  language     = {eng},
  pages        = {373--376},
  title        = {An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers},
  url          = {http://dx.doi.org/10.1109/BioCAS.2011.6107805},
  year         = {2011},
}