An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers
(2011) 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 p.373-376- Abstract
The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/b33c3e7e-65bc-4b5e-9c81-f229b44837ea
- author
- Krishnan, Rajaram ; Charlot, David ; Kumosa, Lucas LU ; Hanna, William ; Lu, Jerry ; Sonnenberg, Avery and Heller, Michael
- publishing date
- 2011
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE
- article number
- 6107805
- pages
- 373 - 376
- conference name
- 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
- conference location
- San Diego, CA, United States
- conference dates
- 2011-11-10 - 2011-11-12
- external identifiers
-
- scopus:84862951501
- ISBN
- 9781457714696
- DOI
- 10.1109/BioCAS.2011.6107805
- language
- English
- LU publication?
- no
- id
- b33c3e7e-65bc-4b5e-9c81-f229b44837ea
- date added to LUP
- 2017-06-23 10:49:22
- date last changed
- 2022-01-30 21:05:42
@inproceedings{b33c3e7e-65bc-4b5e-9c81-f229b44837ea, abstract = {{<p>The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.</p>}}, author = {{Krishnan, Rajaram and Charlot, David and Kumosa, Lucas and Hanna, William and Lu, Jerry and Sonnenberg, Avery and Heller, Michael}}, booktitle = {{Biomedical Circuits and Systems Conference (BioCAS), 2011 IEEE}}, isbn = {{9781457714696}}, language = {{eng}}, pages = {{373--376}}, title = {{An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers}}, url = {{http://dx.doi.org/10.1109/BioCAS.2011.6107805}}, doi = {{10.1109/BioCAS.2011.6107805}}, year = {{2011}}, }