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Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide‑induced acute inflammatory apoM‑/‑ mice

Shi, Yuanping ; Liu, Hongyao ; Liu, Hong ; Yu, Yang ; Zhang, Jun ; Li, Yanfei ; Luo, Guanghua ; Zhang, Xiaoying and Xu, Ning LU (2020) In Molecular Medicine Reports 22(4). p.3117-3126
Abstract

apolipoproteinM(apoM)mayserveaprotectiverole inthedevelopmentofinflammation.Nuclearfactor‑κB(nF-κB) and its downstream factors (including a number of inflammatory cytokines and adhesion molecules) are essential for the regulation of inflammatory processes. In the present study, the importance of apoM in lipopolysaccharide (LPS)‑induced acute inflammation and its potential underlying mechanisms, were investigated using an apoM‑knockout mouse model. The levels of inducible nitric oxide synthase (iNOS), NF‑κB, interleukin (IL)‑1β, intercellular adhesion molecule 1 (ICAM‑1) and vascular cell adhesion protein 1 (VCAM‑1) were detected using reverse transcription‑quantitative PCR and western blotting. The serum levels of IL‑6 and IL‑10 were... (More)

apolipoproteinM(apoM)mayserveaprotectiverole inthedevelopmentofinflammation.Nuclearfactor‑κB(nF-κB) and its downstream factors (including a number of inflammatory cytokines and adhesion molecules) are essential for the regulation of inflammatory processes. In the present study, the importance of apoM in lipopolysaccharide (LPS)‑induced acute inflammation and its potential underlying mechanisms, were investigated using an apoM‑knockout mouse model. The levels of inducible nitric oxide synthase (iNOS), NF‑κB, interleukin (IL)‑1β, intercellular adhesion molecule 1 (ICAM‑1) and vascular cell adhesion protein 1 (VCAM‑1) were detected using reverse transcription‑quantitative PCR and western blotting. The serum levels of IL‑6 and IL‑10 were detected using Luminex technology. The results demonstrated that the protein levels of inoS, nF-κB, il-1β, ICAM‑1 and VCAM‑1 were significantly increased in apoM-/- mice compared with those in apoM+/+ mice. In addition, two‑way ANOVA revealed that the interaction between apoM and LPS had a statistically significant effect on a number of factors, including the mRNA expression levels of hepatic iNOS, NF‑κB, il-1β, icaM-1 and VCAM‑1. Notably, the effects of apoM and 10 mg/kg LPS on the levels of IL‑6 and IL‑10 were the opposite of those induced by 5 mg/kg LPS, which could be associated with the dual anti‑ and pro‑inflammatory effects of IL‑6 and IL‑10. Collectively, the results of the present study revealed that apoM is an important regulator of inflammatory cytokine and adhesion molecule production in LPS‑induced inflammation, which may consequently be associated with the severity of inflammation. These findings indicated that the anti‑inflammatory effects of apoM may partly result from the inhibition of the nF-κB pathway.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adhesion molecules, Anti‑inflammatory cytokines, Apolipoprotein M, Lipopolysaccharide, Pro-inflammatory cytokines
in
Molecular Medicine Reports
volume
22
issue
4
pages
10 pages
publisher
Spandidos Publications
external identifiers
  • scopus:85090175584
  • pmid:32945469
ISSN
1791-2997
DOI
10.3892/mmr.2020.11426
language
English
LU publication?
yes
id
b356f2c3-7cab-43c8-85d0-0f6f1a654cfe
date added to LUP
2020-09-14 14:43:36
date last changed
2022-08-04 00:41:20
@article{b356f2c3-7cab-43c8-85d0-0f6f1a654cfe,
  abstract     = {{<p>apolipoproteinM(apoM)mayserveaprotectiverole inthedevelopmentofinflammation.Nuclearfactor‑κB(nF-κB) and its downstream factors (including a number of inflammatory cytokines and adhesion molecules) are essential for the regulation of inflammatory processes. In the present study, the importance of apoM in lipopolysaccharide (LPS)‑induced acute inflammation and its potential underlying mechanisms, were investigated using an apoM‑knockout mouse model. The levels of inducible nitric oxide synthase (iNOS), NF‑κB, interleukin (IL)‑1β, intercellular adhesion molecule 1 (ICAM‑1) and vascular cell adhesion protein 1 (VCAM‑1) were detected using reverse transcription‑quantitative PCR and western blotting. The serum levels of IL‑6 and IL‑10 were detected using Luminex technology. The results demonstrated that the protein levels of inoS, nF-κB, il-1β, ICAM‑1 and VCAM‑1 were significantly increased in apoM<sup>-/-</sup> mice compared with those in apoM<sup>+/+</sup> mice. In addition, two‑way ANOVA revealed that the interaction between apoM and LPS had a statistically significant effect on a number of factors, including the mRNA expression levels of hepatic iNOS, NF‑κB, il-1β, icaM-1 and VCAM‑1. Notably, the effects of apoM and 10 mg/kg LPS on the levels of IL‑6 and IL‑10 were the opposite of those induced by 5 mg/kg LPS, which could be associated with the dual anti‑ and pro‑inflammatory effects of IL‑6 and IL‑10. Collectively, the results of the present study revealed that apoM is an important regulator of inflammatory cytokine and adhesion molecule production in LPS‑induced inflammation, which may consequently be associated with the severity of inflammation. These findings indicated that the anti‑inflammatory effects of apoM may partly result from the inhibition of the nF-κB pathway.</p>}},
  author       = {{Shi, Yuanping and Liu, Hongyao and Liu, Hong and Yu, Yang and Zhang, Jun and Li, Yanfei and Luo, Guanghua and Zhang, Xiaoying and Xu, Ning}},
  issn         = {{1791-2997}},
  keywords     = {{Adhesion molecules; Anti‑inflammatory cytokines; Apolipoprotein M; Lipopolysaccharide; Pro-inflammatory cytokines}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{3117--3126}},
  publisher    = {{Spandidos Publications}},
  series       = {{Molecular Medicine Reports}},
  title        = {{Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide‑induced acute inflammatory apoM<sup>‑/‑</sup> mice}},
  url          = {{http://dx.doi.org/10.3892/mmr.2020.11426}},
  doi          = {{10.3892/mmr.2020.11426}},
  volume       = {{22}},
  year         = {{2020}},
}