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Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant

Garcia Sanchez, Rosa LU ; Bettiga, Maurizio LU and Gorwa-Grauslund, Marie F LU (2012) In AMB Express 2(1). p.68-68
Abstract
Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could represent a source of Saccharomyces cerevisiae strains with a naturally acquired tolerance towards this inhibitory environment. Two isolation processes were performed, before and after the re-inoculation of the plant with a commercial baker's yeast strain. The isolates were clustered by DNA fingerprinting and a recurrent Saccharomyces cerevisiae... (More)
Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could represent a source of Saccharomyces cerevisiae strains with a naturally acquired tolerance towards this inhibitory environment. Two isolation processes were performed, before and after the re-inoculation of the plant with a commercial baker's yeast strain. The isolates were clustered by DNA fingerprinting and a recurrent Saccharomyces cerevisiae strain, different from the inoculated commercial baker's yeast strain, was isolated. The strain, named TMB3720, flocculated heavily and presented high furaldehyde reductase activity. During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast. Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
AMB Express
volume
2
issue
1
pages
68 - 68
publisher
Springer Open
external identifiers
  • scopus:84908297964
ISSN
2191-0855
DOI
10.1186/2191-0855-2-68
language
English
LU publication?
yes
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b3dd856c-8dd5-4793-80fd-e7751a43f67e
date added to LUP
2019-05-17 14:38:02
date last changed
2020-01-13 01:49:15
@article{b3dd856c-8dd5-4793-80fd-e7751a43f67e,
  abstract     = {Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could represent a source of Saccharomyces cerevisiae strains with a naturally acquired tolerance towards this inhibitory environment. Two isolation processes were performed, before and after the re-inoculation of the plant with a commercial baker's yeast strain. The isolates were clustered by DNA fingerprinting and a recurrent Saccharomyces cerevisiae strain, different from the inoculated commercial baker's yeast strain, was isolated. The strain, named TMB3720, flocculated heavily and presented high furaldehyde reductase activity. During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast. Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest.},
  author       = {Garcia Sanchez, Rosa and Bettiga, Maurizio and Gorwa-Grauslund, Marie F},
  issn         = {2191-0855},
  language     = {eng},
  number       = {1},
  pages        = {68--68},
  publisher    = {Springer Open},
  series       = {AMB Express},
  title        = {Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant},
  url          = {http://dx.doi.org/10.1186/2191-0855-2-68},
  doi          = {10.1186/2191-0855-2-68},
  volume       = {2},
  year         = {2012},
}