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Use of microcalorimetry in analysing the kinetics of ADCC

Ankerst, J. LU orcid ; Sjögren, H. O. LU and Fäldt, R. LU (1986) In Journal of Immunological Methods 88(2). p.259-264
Abstract

Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these... (More)

Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.

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author
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type
Contribution to journal
publication status
published
subject
keywords
Antibody-dependent cellular cytotoxicity, Human lymphocytes, Microcalorimetry, Monoclonal antibody
in
Journal of Immunological Methods
volume
88
issue
2
pages
259 - 264
publisher
Elsevier
external identifiers
  • pmid:3958501
  • scopus:0022652227
ISSN
0022-1759
DOI
10.1016/0022-1759(86)90014-1
language
English
LU publication?
yes
id
b44590c5-01ef-496e-9232-7792e1a65dc1
date added to LUP
2021-01-07 16:04:48
date last changed
2024-01-03 03:07:20
@article{b44590c5-01ef-496e-9232-7792e1a65dc1,
  abstract     = {{<p>Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the G<sub>D3</sub> ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to G<sub>D3</sub> had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.</p>}},
  author       = {{Ankerst, J. and Sjögren, H. O. and Fäldt, R.}},
  issn         = {{0022-1759}},
  keywords     = {{Antibody-dependent cellular cytotoxicity; Human lymphocytes; Microcalorimetry; Monoclonal antibody}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{2}},
  pages        = {{259--264}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{Use of microcalorimetry in analysing the kinetics of ADCC}},
  url          = {{http://dx.doi.org/10.1016/0022-1759(86)90014-1}},
  doi          = {{10.1016/0022-1759(86)90014-1}},
  volume       = {{88}},
  year         = {{1986}},
}