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Determination of microbial fatty acid profiles at femtomolar levels in human urine and the initial marine microfouling community by capillary gas chromatography-chemical ionization mass spectrometry with negative ion detection

Odham, Goran ; Tunlid, Anders LU ; Westerdahl, Gunilla ; Larsson, Lennart LU ; Guckert, James B. and White, David C. (1985) In Journal of Microbiological Methods 3(5-6). p.331-344
Abstract


Room temperature esterification with the electron capturing pentafluorobenzyl bromide in glass capillaries, with analysis by capillary gas-liquid chromatography coupled with chemical ionization mass spectrometry and negative ion detection in the selected ion mode, allowed detection and identification of fatty acids from microbial biofilms at the femtomolar level. This sensitivity was achieved without loss of specificity of the mass spectrometric analysis. The detection of all the fatty acids commonly associated with bacteria was quantitative and linearly related to their concentration at a sensitivity permitting detection of about 600 bacteria the size of Escherichia coli. With this technique it was... (More)


Room temperature esterification with the electron capturing pentafluorobenzyl bromide in glass capillaries, with analysis by capillary gas-liquid chromatography coupled with chemical ionization mass spectrometry and negative ion detection in the selected ion mode, allowed detection and identification of fatty acids from microbial biofilms at the femtomolar level. This sensitivity was achieved without loss of specificity of the mass spectrometric analysis. The detection of all the fatty acids commonly associated with bacteria was quantitative and linearly related to their concentration at a sensitivity permitting detection of about 600 bacteria the size of Escherichia coli. With this technique it was possible to detect the characteristic 3-hydroxy fatty acid of the lipopolysaccharide lipid A of E. coli infecting human urine at concentrations representing 10
4
bacteria and define the community structure of the initial marine microfouling community attached to a teflon surface at concentrations below the detectability by gas chromatography with flame ionization detection.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capillary gas chromatography, Escherichia coli, Fatty acid analysis, Femtomolar sensitivity, Initial fouling community, Negative ion mass spectormetry, Urinary tract infection
in
Journal of Microbiological Methods
volume
3
issue
5-6
pages
331 - 344
publisher
Elsevier
external identifiers
  • scopus:0021814257
ISSN
0167-7012
DOI
10.1016/0167-7012(85)90015-6
language
English
LU publication?
yes
id
b4abdf5d-0724-40a9-b3fb-8b3fa77f5000
date added to LUP
2019-10-23 17:37:13
date last changed
2020-01-13 02:28:49
@article{b4abdf5d-0724-40a9-b3fb-8b3fa77f5000,
  abstract     = {<p><br>
                            Room temperature esterification with the electron capturing pentafluorobenzyl bromide in glass capillaries, with analysis by capillary gas-liquid chromatography coupled with chemical ionization mass spectrometry and negative ion detection in the selected ion mode, allowed detection and identification of fatty acids from microbial biofilms at the femtomolar level. This sensitivity was achieved without loss of specificity of the mass spectrometric analysis. The detection of all the fatty acids commonly associated with bacteria was quantitative and linearly related to their concentration at a sensitivity permitting detection of about 600 bacteria the size of Escherichia coli. With this technique it was possible to detect the characteristic 3-hydroxy fatty acid of the lipopolysaccharide lipid A of E. coli infecting human urine at concentrations representing 10<br>
                            <sup>4</sup><br>
                             bacteria and define the community structure of the initial marine microfouling community attached to a teflon surface at concentrations below the detectability by gas chromatography with flame ionization detection.</p>},
  author       = {Odham, Goran and Tunlid, Anders and Westerdahl, Gunilla and Larsson, Lennart and Guckert, James B. and White, David C.},
  issn         = {0167-7012},
  language     = {eng},
  month        = {01},
  number       = {5-6},
  pages        = {331--344},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Determination of microbial fatty acid profiles at femtomolar levels in human urine and the initial marine microfouling community by capillary gas chromatography-chemical ionization mass spectrometry with negative ion detection},
  url          = {http://dx.doi.org/10.1016/0167-7012(85)90015-6},
  doi          = {10.1016/0167-7012(85)90015-6},
  volume       = {3},
  year         = {1985},
}