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Immunohistochemical markers in full-thickness embryonic rabbit retinal transplants

Ghosh, F LU ; Bruun, A LU and Ehinger, B LU (1999) In Ophthalmic Research 31(1). p.5-15
Abstract

PURPOSE: To examine immunohistochemical markers in straight, well-laminated retinal transplants with special attention paid to the interphotoreceptor matrix, the Müller cells and the ganglion cells as these three retinal components have been abnormal in transplants produced by previous methods.

METHODS: Nine rabbits underwent subretinal transplantation of a complete full-thickness embryonic neuroretina. After 31 or 49 days, the transplants were stained for light microscopy and processed for immunohistochemistry.

RESULTS: Six of 9 eyes contained transplants with straight, well-laminated regions with all light-microscopic characteristics of a normal retina. In the outer segment region, the expression of peanut agglutinin... (More)

PURPOSE: To examine immunohistochemical markers in straight, well-laminated retinal transplants with special attention paid to the interphotoreceptor matrix, the Müller cells and the ganglion cells as these three retinal components have been abnormal in transplants produced by previous methods.

METHODS: Nine rabbits underwent subretinal transplantation of a complete full-thickness embryonic neuroretina. After 31 or 49 days, the transplants were stained for light microscopy and processed for immunohistochemistry.

RESULTS: Six of 9 eyes contained transplants with straight, well-laminated regions with all light-microscopic characteristics of a normal retina. In the outer segment region, the expression of peanut agglutinin showed segmental labeling of cone domains in the interphotoreceptor matrix, and interphotoreceptor retinoid binding protein immunoreactivity was found. Glial fibrillary acidic protein and vimentin immunoreactivity revealed normal Müller cell morphology. In 3 transplants the AB5-antibody-labeled ganglion cells in the ganglion cell layer and all transplants contained nerve fibers in the nerve fiber layer labeled by an antibody against neurofilament of 160 kD. The latter also labeled fibers connecting the transplant with the host.

CONCLUSIONS: Full-thickness embryonic retinal transplants develop the normal retinal appearance and display several of the retinal components necessary for normal function which are not found in transplants produced by previous methods.

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published
subject
keywords
Animals, Biomarkers, Choline O-Acetyltransferase, Eye Proteins, Fetal Tissue Transplantation, Fluorescent Antibody Technique, Indirect, Follow-Up Studies, Glial Fibrillary Acidic Protein, Immunoenzyme Techniques, Photoreceptor Cells, Rabbits, Retina, Retinal Ganglion Cells, Vimentin, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Ophthalmic Research
volume
31
issue
1
pages
5 - 15
publisher
Karger
external identifiers
  • scopus:0032890129
ISSN
0030-3747
DOI
10.1159/000055507
language
English
LU publication?
yes
id
b4bf4bb8-b666-4efd-a420-62670051a122
date added to LUP
2017-05-17 11:38:42
date last changed
2017-06-20 16:17:07
@article{b4bf4bb8-b666-4efd-a420-62670051a122,
  abstract     = {<p>PURPOSE: To examine immunohistochemical markers in straight, well-laminated retinal transplants with special attention paid to the interphotoreceptor matrix, the Müller cells and the ganglion cells as these three retinal components have been abnormal in transplants produced by previous methods.</p><p>METHODS: Nine rabbits underwent subretinal transplantation of a complete full-thickness embryonic neuroretina. After 31 or 49 days, the transplants were stained for light microscopy and processed for immunohistochemistry.</p><p>RESULTS: Six of 9 eyes contained transplants with straight, well-laminated regions with all light-microscopic characteristics of a normal retina. In the outer segment region, the expression of peanut agglutinin showed segmental labeling of cone domains in the interphotoreceptor matrix, and interphotoreceptor retinoid binding protein immunoreactivity was found. Glial fibrillary acidic protein and vimentin immunoreactivity revealed normal Müller cell morphology. In 3 transplants the AB5-antibody-labeled ganglion cells in the ganglion cell layer and all transplants contained nerve fibers in the nerve fiber layer labeled by an antibody against neurofilament of 160 kD. The latter also labeled fibers connecting the transplant with the host.</p><p>CONCLUSIONS: Full-thickness embryonic retinal transplants develop the normal retinal appearance and display several of the retinal components necessary for normal function which are not found in transplants produced by previous methods.</p>},
  author       = {Ghosh, F and Bruun, A and Ehinger, B},
  issn         = {0030-3747},
  keyword      = {Animals,Biomarkers,Choline O-Acetyltransferase,Eye Proteins,Fetal Tissue Transplantation,Fluorescent Antibody Technique, Indirect,Follow-Up Studies,Glial Fibrillary Acidic Protein,Immunoenzyme Techniques,Photoreceptor Cells,Rabbits,Retina,Retinal Ganglion Cells,Vimentin,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {5--15},
  publisher    = {Karger},
  series       = {Ophthalmic Research},
  title        = {Immunohistochemical markers in full-thickness embryonic rabbit retinal transplants},
  url          = {http://dx.doi.org/10.1159/000055507},
  volume       = {31},
  year         = {1999},
}