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Protective effects of selenium on bisphenol a-induced oxidative stress in mouse testicular mitochondria and sperm motility

Rafiee, Zeinab LU ; Rezaee-Tazangi, Fatemeh ; Zeidooni, Leila ; Alidadi, Hadis and Khorsandi, Layasadat (2021) In Jornal Brasileiro de Reproducao Assistida 25(3). p.459-465
Abstract

Objective: This study aimed to explore the impact of selenium (SE) on Bisphenol-A (BPA)-exposed sperm and isolated testicular mitochondria of mice. Methods: Mouse sperm and isolated mitochondria were exposed to BPA (0.8 mM) and different concentrations of SE (50, 100, and 200 µM) for four hours. The viability of sperm and isolated mitochondria as well as the mitochondrial membrane potential (MMP) were evaluated. SOD (superoxide dismutase), GSH (glutathione), MDA (malondialdehyde), and ROS (reactive oxygen species) levels in testicular mitochondria were also examined. Results: BPA concentration-dependently enhanced ROS and MDA levels in isolated mitochondria, while MMP and acclivity of GSH and SOD significantly reduced. BPA also... (More)

Objective: This study aimed to explore the impact of selenium (SE) on Bisphenol-A (BPA)-exposed sperm and isolated testicular mitochondria of mice. Methods: Mouse sperm and isolated mitochondria were exposed to BPA (0.8 mM) and different concentrations of SE (50, 100, and 200 µM) for four hours. The viability of sperm and isolated mitochondria as well as the mitochondrial membrane potential (MMP) were evaluated. SOD (superoxide dismutase), GSH (glutathione), MDA (malondialdehyde), and ROS (reactive oxygen species) levels in testicular mitochondria were also examined. Results: BPA concentration-dependently enhanced ROS and MDA levels in isolated mitochondria, while MMP and acclivity of GSH and SOD significantly reduced. BPA also considerably impaired spermatozoa survival and motility. SE concentration-dependently reduced mitochondrial oxidative stress, MMP, sperm survival, and total sperm motility. Conclusions: Our findings collectively suggested that SE concentration-dependently reversed BPA-caused mitochondrial toxicity and reduced sperm motility by suppressing oxidative stress.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bisphenol A, Oxidative stress, Selenium, Sperm motility
in
Jornal Brasileiro de Reproducao Assistida
volume
25
issue
3
pages
459 - 465
publisher
Sociedade Brasileira de Reproducao Assistida
external identifiers
  • pmid:33899458
  • scopus:85111143337
ISSN
1517-5693
DOI
10.5935/1518-0557.20210010
language
English
LU publication?
no
additional info
Funding Information: Ahvaz Jundishapur University Student Research committee supported this study (Grant No: 97s-26). Publisher Copyright: © 2021, Sociedade Brasileira de Reproducao Assistida. All rights reserved. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
id
b52860a8-b333-402e-bbdc-5611b623c155
date added to LUP
2021-09-23 20:42:38
date last changed
2024-06-15 16:49:28
@article{b52860a8-b333-402e-bbdc-5611b623c155,
  abstract     = {{<p>Objective: This study aimed to explore the impact of selenium (SE) on Bisphenol-A (BPA)-exposed sperm and isolated testicular mitochondria of mice. Methods: Mouse sperm and isolated mitochondria were exposed to BPA (0.8 mM) and different concentrations of SE (50, 100, and 200 µM) for four hours. The viability of sperm and isolated mitochondria as well as the mitochondrial membrane potential (MMP) were evaluated. SOD (superoxide dismutase), GSH (glutathione), MDA (malondialdehyde), and ROS (reactive oxygen species) levels in testicular mitochondria were also examined. Results: BPA concentration-dependently enhanced ROS and MDA levels in isolated mitochondria, while MMP and acclivity of GSH and SOD significantly reduced. BPA also considerably impaired spermatozoa survival and motility. SE concentration-dependently reduced mitochondrial oxidative stress, MMP, sperm survival, and total sperm motility. Conclusions: Our findings collectively suggested that SE concentration-dependently reversed BPA-caused mitochondrial toxicity and reduced sperm motility by suppressing oxidative stress.</p>}},
  author       = {{Rafiee, Zeinab and Rezaee-Tazangi, Fatemeh and Zeidooni, Leila and Alidadi, Hadis and Khorsandi, Layasadat}},
  issn         = {{1517-5693}},
  keywords     = {{Bisphenol A; Oxidative stress; Selenium; Sperm motility}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{459--465}},
  publisher    = {{Sociedade Brasileira de Reproducao Assistida}},
  series       = {{Jornal Brasileiro de Reproducao Assistida}},
  title        = {{Protective effects of selenium on bisphenol a-induced oxidative stress in mouse testicular mitochondria and sperm motility}},
  url          = {{http://dx.doi.org/10.5935/1518-0557.20210010}},
  doi          = {{10.5935/1518-0557.20210010}},
  volume       = {{25}},
  year         = {{2021}},
}