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Upregulation of endothelin ETB receptor-mediated vasoconstriction in rat coronary artery after organ culture

Eskesen, K and Edvinsson, Lars LU (2006) In European Journal of Pharmacology 539(3). p.192-194
Abstract
The aim of this study was to examine if endothelin ETB receptor-mediated contraction occurred in isolated segments of rat coronary arteries during organ culture. Presence of contractile endothelin ETB receptors was studied by measuring the change in isometric tension in rings of left anterior descending coronary arteries isolated from hearts of rats as response to application of the selective endothelin ETB receptor agonist, Sarafotoxin 6c and endothelin-1. In segments cultured 1 day in serum free Dulbecco's Modified Eagle's Medium, Sarafotoxin 6c induced a concentration dependent contraction with pEC(50) value of 10.4 +/- 0.21 and a maximal response of 3.9 +/- 0.25 mN/mm (n = 15). The maximal contraction was significantly larger than the... (More)
The aim of this study was to examine if endothelin ETB receptor-mediated contraction occurred in isolated segments of rat coronary arteries during organ culture. Presence of contractile endothelin ETB receptors was studied by measuring the change in isometric tension in rings of left anterior descending coronary arteries isolated from hearts of rats as response to application of the selective endothelin ETB receptor agonist, Sarafotoxin 6c and endothelin-1. In segments cultured 1 day in serum free Dulbecco's Modified Eagle's Medium, Sarafotoxin 6c induced a concentration dependent contraction with pEC(50) value of 10.4 +/- 0.21 and a maximal response of 3.9 +/- 0.25 mN/mm (n = 15). The maximal contraction was significantly larger than the responses measured in fresh tissue, where the mean value of the maximal contractions was 0.22 +/- 0.03 mN/mm (n = 17). The increased contraction to Sarafotoxin 6c, after culturing could be eliminated with addition of the transcriptional blocker, actinomycin D, to the culture medium or be significantly attenuated by application of the translational inhibitor, cycloheximide. The vasoconstrictor effect of endothelin-1 or to depolarisation by high K+-solution was not modified after 1 day in culture medium. The experiments indicate that organ culture of rat coronary arteries upregulate endothelin ETB receptor-mediated contraction by inducing synthesis of new protein. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cycloheximide, Sarafotoxin 6c, actinomycin D, vasoconstriction
in
European Journal of Pharmacology
volume
539
issue
3
pages
192 - 194
publisher
Elsevier
external identifiers
  • pmid:16725137
  • wos:000238346100008
  • scopus:33744459253
ISSN
1879-0712
DOI
10.1016/j.ejphar.2006.04.024
language
English
LU publication?
yes
id
b53dd734-d576-42ed-8e0f-a70425b980ab (old id 406118)
date added to LUP
2007-10-02 18:44:01
date last changed
2019-02-20 04:14:38
@article{b53dd734-d576-42ed-8e0f-a70425b980ab,
  abstract     = {The aim of this study was to examine if endothelin ETB receptor-mediated contraction occurred in isolated segments of rat coronary arteries during organ culture. Presence of contractile endothelin ETB receptors was studied by measuring the change in isometric tension in rings of left anterior descending coronary arteries isolated from hearts of rats as response to application of the selective endothelin ETB receptor agonist, Sarafotoxin 6c and endothelin-1. In segments cultured 1 day in serum free Dulbecco's Modified Eagle's Medium, Sarafotoxin 6c induced a concentration dependent contraction with pEC(50) value of 10.4 +/- 0.21 and a maximal response of 3.9 +/- 0.25 mN/mm (n = 15). The maximal contraction was significantly larger than the responses measured in fresh tissue, where the mean value of the maximal contractions was 0.22 +/- 0.03 mN/mm (n = 17). The increased contraction to Sarafotoxin 6c, after culturing could be eliminated with addition of the transcriptional blocker, actinomycin D, to the culture medium or be significantly attenuated by application of the translational inhibitor, cycloheximide. The vasoconstrictor effect of endothelin-1 or to depolarisation by high K+-solution was not modified after 1 day in culture medium. The experiments indicate that organ culture of rat coronary arteries upregulate endothelin ETB receptor-mediated contraction by inducing synthesis of new protein. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Eskesen, K and Edvinsson, Lars},
  issn         = {1879-0712},
  keyword      = {cycloheximide,Sarafotoxin 6c,actinomycin D,vasoconstriction},
  language     = {eng},
  number       = {3},
  pages        = {192--194},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Upregulation of endothelin ETB receptor-mediated vasoconstriction in rat coronary artery after organ culture},
  url          = {http://dx.doi.org/10.1016/j.ejphar.2006.04.024},
  volume       = {539},
  year         = {2006},
}