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Interaction of polymer-coated gold nanoparticles with cellobiose dehydrogenase : The role of surface charges

Tavahodi, Mojtaba; Schulz, Christopher LU ; Assarsson, Anna LU ; Ortiz, Roberto LU ; Ludwig, Roland; Cabaleiro-Lago, Celia LU ; Haghighi, Behzad LU and Gorton, Lo LU (2017) In Journal of Electroanalytical Chemistry
Abstract

Studying the interaction of functional proteins such as enzymes and nanoparticles (NPs) includes the important topic of investigating any possible changes in stability and function of enzymes in nanostructured environments. The effects of NPs on the enzyme activity and stability are governed by their physical and chemical properties such as structure, shape, size, surface chemistry and their surface charges. In this study, the influence of negatively and positively charged AuNPs are investigated on the activity of immobilized Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) and its electron transfer rate with graphite electrodes modified with positively and negatively AuNPs. The MtCDH modified graphite electrode premodified... (More)

Studying the interaction of functional proteins such as enzymes and nanoparticles (NPs) includes the important topic of investigating any possible changes in stability and function of enzymes in nanostructured environments. The effects of NPs on the enzyme activity and stability are governed by their physical and chemical properties such as structure, shape, size, surface chemistry and their surface charges. In this study, the influence of negatively and positively charged AuNPs are investigated on the activity of immobilized Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) and its electron transfer rate with graphite electrodes modified with positively and negatively AuNPs. The MtCDH modified graphite electrode premodified with positively charged AuNPs showed an alkaline shift in the pH of maximum activity from pH5.5 to 8. No change in the pH of maximum activity was observed when MtCDH graphite electrodes were premodified with negatively charged AuNPs. The results clearly demonstrated the effect of surface charge of AuNPs on the activity of the enzyme. The catalytic current density and the KM app value for MtCDH graphite electrode premodified with positively charged AuNPs were enhanced with up to 66 and 8 times, respectively. Two spectroscopic assays were also performed in solution to investigate the influence of the presence of positively or negatively charged AuNPs on the activity of MtCDH in homogeneous solution. The results clearly demonstrated that not only the rate of the heterogeneous electron transfer between the immobilized MtCDH and the electrode but also the rate of the homogeneous electron transfer between soluble MtCDH and the acceptor was highly dependent on the type of surface charge of the AuNPs.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Biofuel cell, Biosensor, Direct electron transfer
in
Journal of Electroanalytical Chemistry
publisher
Elsevier
external identifiers
  • scopus:85031815820
ISSN
1572-6657
DOI
10.1016/j.jelechem.2017.10.035
language
English
LU publication?
yes
id
b57c3621-0322-47d5-949b-98ec01ad8084
date added to LUP
2017-10-31 11:01:16
date last changed
2018-01-07 12:24:28
@article{b57c3621-0322-47d5-949b-98ec01ad8084,
  abstract     = {<p>Studying the interaction of functional proteins such as enzymes and nanoparticles (NPs) includes the important topic of investigating any possible changes in stability and function of enzymes in nanostructured environments. The effects of NPs on the enzyme activity and stability are governed by their physical and chemical properties such as structure, shape, size, surface chemistry and their surface charges. In this study, the influence of negatively and positively charged AuNPs are investigated on the activity of immobilized Myriococcum thermophilum cellobiose dehydrogenase (MtCDH) and its electron transfer rate with graphite electrodes modified with positively and negatively AuNPs. The MtCDH modified graphite electrode premodified with positively charged AuNPs showed an alkaline shift in the pH of maximum activity from pH5.5 to 8. No change in the pH of maximum activity was observed when MtCDH graphite electrodes were premodified with negatively charged AuNPs. The results clearly demonstrated the effect of surface charge of AuNPs on the activity of the enzyme. The catalytic current density and the K<sub>M</sub> <sup>app</sup> value for MtCDH graphite electrode premodified with positively charged AuNPs were enhanced with up to 66 and 8 times, respectively. Two spectroscopic assays were also performed in solution to investigate the influence of the presence of positively or negatively charged AuNPs on the activity of MtCDH in homogeneous solution. The results clearly demonstrated that not only the rate of the heterogeneous electron transfer between the immobilized MtCDH and the electrode but also the rate of the homogeneous electron transfer between soluble MtCDH and the acceptor was highly dependent on the type of surface charge of the AuNPs.</p>},
  author       = {Tavahodi, Mojtaba and Schulz, Christopher and Assarsson, Anna and Ortiz, Roberto and Ludwig, Roland and Cabaleiro-Lago, Celia and Haghighi, Behzad and Gorton, Lo},
  issn         = {1572-6657},
  keyword      = {Biofuel cell,Biosensor,Direct electron transfer},
  language     = {eng},
  month        = {10},
  publisher    = {Elsevier},
  series       = {Journal of Electroanalytical Chemistry},
  title        = {Interaction of polymer-coated gold nanoparticles with cellobiose dehydrogenase : The role of surface charges},
  url          = {http://dx.doi.org/10.1016/j.jelechem.2017.10.035},
  year         = {2017},
}