In vitro evidence of root colonization suggests ecological versatility in the genus Mycena
(2020) In New Phytologist 227(2). p.601-612- Abstract
The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. In all, 17 Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were... (More)
The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. In all, 17 Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were investigated using 14 C and 32 P to show potential transfer between symbionts. All Mycena species associated closely with fine roots, showing hyphal penetration into the roots, which in some cases were intracellular. Seven species formed mantle-like structures around root tips, but none formed a Hartig net. Mycena pura and Mycena galopus both enhanced seedling growth, with M. pura showing significant transfer of 32 P to the seedlings. Our results support the view that several Mycena species can associate closely with plant roots and some may potentially occupy a transitional state between saprotrophy and biotrophy.
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- author
- Thoen, Ella ; Harder, Christoffer Bugge ; Kauserud, Håvard ; Botnen, Synnøve S ; Vik, Unni ; Taylor, Andy F S ; Menkis, Audrius and Skrede, Inger
- publishing date
- 2020-07
- type
- Contribution to journal
- publication status
- published
- in
- New Phytologist
- volume
- 227
- issue
- 2
- pages
- 601 - 612
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:32171021
- scopus:85083335204
- ISSN
- 1469-8137
- DOI
- 10.1111/nph.16545
- language
- English
- LU publication?
- no
- additional info
- © 2020 The Authors. New Phytologist © 2020 New Phytologist Trust.
- id
- b5af1a3c-4b90-4a89-baf9-803c0c6f18ae
- date added to LUP
- 2020-09-09 11:13:25
- date last changed
- 2024-06-13 22:56:30
@article{b5af1a3c-4b90-4a89-baf9-803c0c6f18ae,
abstract = {{<p>The root-associated habit has evolved on numerous occasions in different fungal lineages, suggesting a strong evolutionary pressure for saprotrophic fungi to switch to symbiotic associations with plants. Species within the ubiquitous, saprotrophic genus Mycena are frequently major components in molecular studies of root-associated fungal communities, suggesting that an evaluation of their trophic status is warranted. Here, we report on interactions between a range of Mycena species and the plant Betula pendula. In all, 17 Mycena species were inoculated onto B. pendula seedlings. Physical interactions between hyphae and fine roots were examined using differential staining and fluorescence microscopy. Physiological interactions were investigated using 14 C and 32 P to show potential transfer between symbionts. All Mycena species associated closely with fine roots, showing hyphal penetration into the roots, which in some cases were intracellular. Seven species formed mantle-like structures around root tips, but none formed a Hartig net. Mycena pura and Mycena galopus both enhanced seedling growth, with M. pura showing significant transfer of 32 P to the seedlings. Our results support the view that several Mycena species can associate closely with plant roots and some may potentially occupy a transitional state between saprotrophy and biotrophy.</p>}},
author = {{Thoen, Ella and Harder, Christoffer Bugge and Kauserud, Håvard and Botnen, Synnøve S and Vik, Unni and Taylor, Andy F S and Menkis, Audrius and Skrede, Inger}},
issn = {{1469-8137}},
language = {{eng}},
number = {{2}},
pages = {{601--612}},
publisher = {{Wiley-Blackwell}},
series = {{New Phytologist}},
title = {{In vitro evidence of root colonization suggests ecological versatility in the genus Mycena}},
url = {{http://dx.doi.org/10.1111/nph.16545}},
doi = {{10.1111/nph.16545}},
volume = {{227}},
year = {{2020}},
}