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Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain.

Schéele, Susanne LU ; Sasaki, Takako; Arnal-Estapé, Anna; Durbeej-Hjalt, Madeleine LU and Ekblom, Peter LU (2006) In Matrix Biology 25(5). p.301-305
Abstract
We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-a1 beta 1 gamma 1 (laminin-11), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and inummostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha 1 chain. AL-4 has also been marketed as an alpha 1 chain specific probe, but we show here that AL-4 detects mouse laminin beta 1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta 1 chain. (c) 2006 Elsevier B.V./Intemational Society of Matrix Biology. All rights reserved.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
kidney, muscle, recombinant proteins, monoclonal antibodies, laminin, ELISA
in
Matrix Biology
volume
25
issue
5
pages
301 - 305
publisher
Elsevier
external identifiers
  • wos:000239028800005
  • pmid:16631359
  • scopus:33745454929
ISSN
1569-1802
DOI
10.1016/j.matbio.2006.03.004
language
English
LU publication?
yes
id
b5d05b5d-9c23-4f3f-b48e-b8f24f5ba50f (old id 155801)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16631359&dopt=Abstract
date added to LUP
2007-07-19 12:38:18
date last changed
2019-02-20 08:07:46
@article{b5d05b5d-9c23-4f3f-b48e-b8f24f5ba50f,
  abstract     = {We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-a1 beta 1 gamma 1 (laminin-11), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and inummostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha 1 chain. AL-4 has also been marketed as an alpha 1 chain specific probe, but we show here that AL-4 detects mouse laminin beta 1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta 1 chain. (c) 2006 Elsevier B.V./Intemational Society of Matrix Biology. All rights reserved.},
  author       = {Schéele, Susanne and Sasaki, Takako and Arnal-Estapé, Anna and Durbeej-Hjalt, Madeleine and Ekblom, Peter},
  issn         = {1569-1802},
  keyword      = {kidney,muscle,recombinant proteins,monoclonal antibodies,laminin,ELISA},
  language     = {eng},
  number       = {5},
  pages        = {301--305},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Monoclonal anti-mouse laminin antibodies: AL-1 reacts with laminin alpha1 chain, AL-2 with laminin beta1 chain, and AL-4 with the coiled-coil domain of laminin beta1 chain.},
  url          = {http://dx.doi.org/10.1016/j.matbio.2006.03.004},
  volume       = {25},
  year         = {2006},
}