Do type 1 fimbriae promote inflammation in the human urinary tract?
(2007) In Cellular Microbiology 9(7). p.1766-1781- Abstract
- Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using... (More)
- Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using cytokines and neutrophils as end-points. Type 1 fimbriae did not promote inflammation and adherence was poor, as examined on exfoliated cells in urine. This was unexpected, as type 1 fimbriae enhanced the inflammatory response to the same strain in the murine urinary tract and as P fimbrial expression by E. coli 83972 enhances adherence and inflammation in challenged patients. We conclude that type 1 fimbriae do not contribute to the mucosal inflammatory response in the human urinary tract. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/166574
- author
- Bergsten, Göran LU ; Wullt, Björn LU ; Schembri, Mark A ; Leijonhufvud, Irene LU and Svanborg, Catharina LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cellular Microbiology
- volume
- 9
- issue
- 7
- pages
- 1766 - 1781
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000247319000014
- scopus:34250698573
- pmid:17359236
- ISSN
- 1462-5814
- DOI
- 10.1111/j.1462-5822.2007.00912.x
- language
- English
- LU publication?
- yes
- id
- b5d193d1-b728-4d60-beb3-482926d9ef55 (old id 166574)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17359236&dopt=Abstract
- date added to LUP
- 2016-04-01 12:07:47
- date last changed
- 2022-03-13 05:44:47
@article{b5d193d1-b728-4d60-beb3-482926d9ef55,
abstract = {{Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using cytokines and neutrophils as end-points. Type 1 fimbriae did not promote inflammation and adherence was poor, as examined on exfoliated cells in urine. This was unexpected, as type 1 fimbriae enhanced the inflammatory response to the same strain in the murine urinary tract and as P fimbrial expression by E. coli 83972 enhances adherence and inflammation in challenged patients. We conclude that type 1 fimbriae do not contribute to the mucosal inflammatory response in the human urinary tract.}},
author = {{Bergsten, Göran and Wullt, Björn and Schembri, Mark A and Leijonhufvud, Irene and Svanborg, Catharina}},
issn = {{1462-5814}},
language = {{eng}},
number = {{7}},
pages = {{1766--1781}},
publisher = {{Wiley-Blackwell}},
series = {{Cellular Microbiology}},
title = {{Do type 1 fimbriae promote inflammation in the human urinary tract?}},
url = {{http://dx.doi.org/10.1111/j.1462-5822.2007.00912.x}},
doi = {{10.1111/j.1462-5822.2007.00912.x}},
volume = {{9}},
year = {{2007}},
}