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Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol

Murillo, Jimmy Rodriguez; Pla, Indira LU ; Goto-Silva, Livia; Nogueira, Fábio C.S.; Domont, Gilberto B.; Perez-Riverol, Yasset; Sánchez, Aniel LU and Junqueira, Magno (2018) In Analytical Biochemistry 559. p.51-54
Abstract

Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) < 20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biological variation, Cell culture, iTRAQ, Quantitative proteomics
in
Analytical Biochemistry
volume
559
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85052069370
ISSN
0003-2697
DOI
10.1016/j.ab.2018.08.013
language
English
LU publication?
yes
id
b60c6fba-b303-465f-86e7-a10e326ed169
date added to LUP
2018-09-25 08:37:03
date last changed
2019-01-06 14:05:35
@article{b60c6fba-b303-465f-86e7-a10e326ed169,
  abstract     = {<p>Cell line-based proteomics studies are susceptible to intrinsic biological variation that contributes to increasing false positive claims; most of the methods that follow these changes offer a limited understanding of the biological system. We applied a quantitative proteomic strategy (iTRAQ) to detect intrinsic protein variation across SH-SY5Y cell culture replicates. More than 95% of the quantified proteins presented a coefficient of variation (CV) &lt; 20% between biological replicates and the variable proteins, which included cytoskeleton, cytoplasmic and housekeeping proteins, are widely reported in proteomic studies. We recommend this approach as an additional quality control before starting any proteomic experiment.</p>},
  author       = {Murillo, Jimmy Rodriguez and Pla, Indira and Goto-Silva, Livia and Nogueira, Fábio C.S. and Domont, Gilberto B. and Perez-Riverol, Yasset and Sánchez, Aniel and Junqueira, Magno},
  issn         = {0003-2697},
  keyword      = {Biological variation,Cell culture,iTRAQ,Quantitative proteomics},
  language     = {eng},
  month        = {10},
  pages        = {51--54},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {Mass spectrometry evaluation of a neuroblastoma SH-SY5Y cell culture protocol},
  url          = {http://dx.doi.org/10.1016/j.ab.2018.08.013},
  volume       = {559},
  year         = {2018},
}