Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid

Schwieler, Lilly ; Trepci, Ada ; Krzyzanowski, Stanislaw ; Hermansson, Sigurd ; Granqvist, Mathias ; Piehl, Fredrik ; Venckunas, Tomas ; Brazaitis, Marius ; Kamandulis, Sigitas and Lindqvist, Daniel LU , et al. (2020) In Bioanalysis 12(6). p.379-392
Abstract

Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated,... (More)

Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
LC-MS/MS, nicotinic acid, picolinic acid, quinolinic acid, suicide, tryptophan-kynurenine pathway
in
Bioanalysis
volume
12
issue
6
pages
14 pages
publisher
Future Science
external identifiers
  • scopus:85084027249
  • pmid:32209024
ISSN
1757-6180
DOI
10.4155/bio-2019-0303
language
English
LU publication?
yes
id
b652c0a7-cd57-427e-84d2-5489d5d86522
date added to LUP
2020-05-14 16:01:11
date last changed
2024-06-12 13:35:22
@article{b652c0a7-cd57-427e-84d2-5489d5d86522,
  abstract     = {{<p>Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology &amp; results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.</p>}},
  author       = {{Schwieler, Lilly and Trepci, Ada and Krzyzanowski, Stanislaw and Hermansson, Sigurd and Granqvist, Mathias and Piehl, Fredrik and Venckunas, Tomas and Brazaitis, Marius and Kamandulis, Sigitas and Lindqvist, Daniel and Jones, Arthur Daniel and Erhardt, Sophie and Brundin, Lena}},
  issn         = {{1757-6180}},
  keywords     = {{LC-MS/MS; nicotinic acid; picolinic acid; quinolinic acid; suicide; tryptophan-kynurenine pathway}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{6}},
  pages        = {{379--392}},
  publisher    = {{Future Science}},
  series       = {{Bioanalysis}},
  title        = {{A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid}},
  url          = {{http://dx.doi.org/10.4155/bio-2019-0303}},
  doi          = {{10.4155/bio-2019-0303}},
  volume       = {{12}},
  year         = {{2020}},
}