A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid
(2020) In Bioanalysis 12(6). p.379-392- Abstract
Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated,... (More)
Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.
(Less)
- author
- organization
- publishing date
- 2020-03-25
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- LC-MS/MS, nicotinic acid, picolinic acid, quinolinic acid, suicide, tryptophan-kynurenine pathway
- in
- Bioanalysis
- volume
- 12
- issue
- 6
- pages
- 14 pages
- publisher
- Future Science
- external identifiers
-
- scopus:85084027249
- pmid:32209024
- ISSN
- 1757-6180
- DOI
- 10.4155/bio-2019-0303
- language
- English
- LU publication?
- yes
- id
- b652c0a7-cd57-427e-84d2-5489d5d86522
- date added to LUP
- 2020-05-14 16:01:11
- date last changed
- 2024-09-18 22:50:12
@article{b652c0a7-cd57-427e-84d2-5489d5d86522, abstract = {{<p>Aim: Kynurenine metabolites are potential modulators of psychiatric disease. We aimed to develop a highly sensitive biochemical analysis of cerebrospinal fluid (CSF) tryptophan (TRP) metabolites, to investigate the stability of metabolites and to confirm our previous findings of aberrant CSF quinolinic acid (QUIN) and picolinic acid (PIC) in suicide attempters using this method. Methodology & results: Ten CSF TRP metabolites were analyzed with ultraperformance LC-MS/MS. The method showed small intra- and interassay variation. Metabolites were stable following freeze-thaw cycles. A decreased CSF PIC/QUIN ratio was found in suicide attempters. Conclusion: The feasibility of reliably determining CSF TRP metabolites were demonstrated, including separation of the two isomers PIC and nicotinic acid (NA) and the finding of a reduced PIC/QUIN ratio replicated in suicide attempters.</p>}}, author = {{Schwieler, Lilly and Trepci, Ada and Krzyzanowski, Stanislaw and Hermansson, Sigurd and Granqvist, Mathias and Piehl, Fredrik and Venckunas, Tomas and Brazaitis, Marius and Kamandulis, Sigitas and Lindqvist, Daniel and Jones, Arthur Daniel and Erhardt, Sophie and Brundin, Lena}}, issn = {{1757-6180}}, keywords = {{LC-MS/MS; nicotinic acid; picolinic acid; quinolinic acid; suicide; tryptophan-kynurenine pathway}}, language = {{eng}}, month = {{03}}, number = {{6}}, pages = {{379--392}}, publisher = {{Future Science}}, series = {{Bioanalysis}}, title = {{A novel, robust method for quantification of multiple kynurenine pathway metabolites in the cerebrospinal fluid}}, url = {{http://dx.doi.org/10.4155/bio-2019-0303}}, doi = {{10.4155/bio-2019-0303}}, volume = {{12}}, year = {{2020}}, }