Analysis of islet cell antibodies on frozen sections of human pancreas
Marner, B. ; Lernmark, Å LU
; Nerup, J.
LU
; Molenaar, J. L.
; Tuk, C. W.
and Bruining, G. J.
(1983)
In Diabetologia
25(2).
p.93-96
- Abstract
The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p < 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of... (More)
The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p < 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of frozen sections, in the indirect immunofluorescence assay.
(Less)
- author
- Marner, B.
; Lernmark, Å
LU
; Nerup, J.
LU
; Molenaar, J. L.
; Tuk, C. W.
and Bruining, G. J.
- publishing date
- 1983-08-01
- type
- Contribution to journal
- publication status
- published
- keywords
- assay precision, assay reproducibility, indirect immunofluorescence, insulin-dependent diabetes, Islet cell antibodies
- in
- Diabetologia
- volume
- 25
- issue
- 2
- pages
- 4 pages
- publisher
- Springer
- external identifiers
-
- scopus:0020594205
- pmid:6354815
- ISSN
- 0012-186X
- DOI
- 10.1007/BF00250895
- language
- English
- LU publication?
- no
- id
- b6ccf7df-8700-4af7-a42b-6bb2a0bf80ae
- date added to LUP
- 2019-09-16 12:49:39
- date last changed
- 2024-03-13 08:04:34
@article{b6ccf7df-8700-4af7-a42b-6bb2a0bf80ae,
abstract = {{<p>The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p < 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of frozen sections, in the indirect immunofluorescence assay.</p>}},
author = {{Marner, B. and Lernmark, Å and Nerup, J. and Molenaar, J. L. and Tuk, C. W. and Bruining, G. J.}},
issn = {{0012-186X}},
keywords = {{assay precision; assay reproducibility; indirect immunofluorescence; insulin-dependent diabetes; Islet cell antibodies}},
language = {{eng}},
month = {{08}},
number = {{2}},
pages = {{93--96}},
publisher = {{Springer}},
series = {{Diabetologia}},
title = {{Analysis of islet cell antibodies on frozen sections of human pancreas}},
url = {{http://dx.doi.org/10.1007/BF00250895}},
doi = {{10.1007/BF00250895}},
volume = {{25}},
year = {{1983}},
}