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Analysis of C3 nephritic factors by ELISA

Skattum, Lillemor LU (2019) In Methods in Molecular Biology 1901. p.177-182
Abstract

Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the circulation and production of C3 degradation fragments due to prolonged stabilization of the C3 convertase. C3NeF are associated with glomerulopathy, acquired partial lipodystrophy, and less frequently with increased susceptibility to meningococcal infection. Analysis of C3NeF is an important part of the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Therefore it is recommended to use a combination of different analysis methods for the detection of C3NeF. Here we present an ELISA method for detection of C3NeF.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Alternative pathway convertase, Autoantibodies to complement, C3 glomerulopathy, C3 nephritic factors, ELISA
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1901
pages
6 pages
publisher
Humana Press
external identifiers
  • scopus:85058613120
  • pmid:30539576
ISSN
1064-3745
DOI
10.1007/978-1-4939-8949-2_13
language
English
LU publication?
yes
id
b7070d1f-b289-4c9d-a8df-6e975aae762c
date added to LUP
2019-01-02 14:29:54
date last changed
2019-12-10 07:51:59
@inbook{b7070d1f-b289-4c9d-a8df-6e975aae762c,
  abstract     = {<p>Autoantibodies to the C3 convertase of the alternative pathway of complement, called C3 nephritic factors (C3NeF), cause persistently low C3 in the circulation and production of C3 degradation fragments due to prolonged stabilization of the C3 convertase. C3NeF are associated with glomerulopathy, acquired partial lipodystrophy, and less frequently with increased susceptibility to meningococcal infection. Analysis of C3NeF is an important part of the diagnostic workup of C3 glomerulopathy, but their identification is difficult presumably due to considerable heterogeneity. Therefore it is recommended to use a combination of different analysis methods for the detection of C3NeF. Here we present an ELISA method for detection of C3NeF.</p>},
  author       = {Skattum, Lillemor},
  booktitle    = {Methods in Molecular Biology},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {177--182},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Analysis of C3 nephritic factors by ELISA},
  url          = {http://dx.doi.org/10.1007/978-1-4939-8949-2_13},
  doi          = {10.1007/978-1-4939-8949-2_13},
  volume       = {1901},
  year         = {2019},
}