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Bioreduction

Skorupa Parachin, Nádia LU ; Carlquist, Magnus LU and Gorwa-Grauslund, Marie-F LU (2010)
Abstract
Bioreduction has emerged over the years as an alternative method to organic synthesis for the generation of chiral precursors of commercial interest. Bioreductions operate under mild conditions of pH and temperature with the help of highly regio- and enantio-selective oxidoreductase enzymes.

In this contribution, the different oxidoreductase families involved in bioreductions are exemplified and their main characteristics are presented. The wide spectrum of oxidoreductase substrates (including ketones, diketones, ketoesters, aldehydes, alkenes, and keto acids) is discussed and both preparative and industrial scale examples are reported. The advantages and disadvantages of using isolated enzymatic systems versus whole-cell systems... (More)
Bioreduction has emerged over the years as an alternative method to organic synthesis for the generation of chiral precursors of commercial interest. Bioreductions operate under mild conditions of pH and temperature with the help of highly regio- and enantio-selective oxidoreductase enzymes.

In this contribution, the different oxidoreductase families involved in bioreductions are exemplified and their main characteristics are presented. The wide spectrum of oxidoreductase substrates (including ketones, diketones, ketoesters, aldehydes, alkenes, and keto acids) is discussed and both preparative and industrial scale examples are reported. The advantages and disadvantages of using isolated enzymatic systems versus whole-cell systems for bioreduction are discussed in terms of cost, specificity, stereoselectivity, and cofactor regeneration. The contribution is also reviewing strategies for improving the biocatalyst at the cell or enzyme level, which include process engineering, metabolic engineering as well as structure-based and nonstructure-based enzyme engineering. Finally, the potential role of metagenomics for isolating novel biocatalysts from different environments is discussed. (Less)
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author
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organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Enclyclopedia of Industrial Biotechnology : Bioprocess, Bioseparation and Cell Technology - Bioprocess, Bioseparation and Cell Technology
editor
Flickinger, Michael C.
pages
19 pages
publisher
Wiley
ISBN
9780470054581
9780471799306
DOI
10.1002/9780470054581.eib151
language
English
LU publication?
yes
id
b721b125-0108-4a17-b490-cace371d6d69
date added to LUP
2019-05-17 14:50:28
date last changed
2022-06-15 09:39:31
@inbook{b721b125-0108-4a17-b490-cace371d6d69,
  abstract     = {{Bioreduction has emerged over the years as an alternative method to organic synthesis for the generation of chiral precursors of commercial interest. Bioreductions operate under mild conditions of pH and temperature with the help of highly regio- and enantio-selective oxidoreductase enzymes.<br/><br/>In this contribution, the different oxidoreductase families involved in bioreductions are exemplified and their main characteristics are presented. The wide spectrum of oxidoreductase substrates (including ketones, diketones, ketoesters, aldehydes, alkenes, and keto acids) is discussed and both preparative and industrial scale examples are reported. The advantages and disadvantages of using isolated enzymatic systems versus whole-cell systems for bioreduction are discussed in terms of cost, specificity, stereoselectivity, and cofactor regeneration. The contribution is also reviewing strategies for improving the biocatalyst at the cell or enzyme level, which include process engineering, metabolic engineering as well as structure-based and nonstructure-based enzyme engineering. Finally, the potential role of metagenomics for isolating novel biocatalysts from different environments is discussed.}},
  author       = {{Skorupa Parachin, Nádia and Carlquist, Magnus and Gorwa-Grauslund, Marie-F}},
  booktitle    = {{Enclyclopedia of Industrial Biotechnology : Bioprocess, Bioseparation and Cell Technology}},
  editor       = {{Flickinger, Michael C.}},
  isbn         = {{9780470054581}},
  language     = {{eng}},
  publisher    = {{Wiley}},
  title        = {{Bioreduction}},
  url          = {{http://dx.doi.org/10.1002/9780470054581.eib151}},
  doi          = {{10.1002/9780470054581.eib151}},
  year         = {{2010}},
}