Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle

Clyne, N. LU orcid ; Wibom, R. ; Havu, N. ; Hultman, E. ; Pehrsson, S. K. and Persson, B. (1990) In Scandinavian Journal of Clinical & Laboratory Investigation 50(2). p.153-159
Abstract

Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The... (More)

Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO4 7 H2O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Cobalt, Mitochondrial ATP-production, Rat myocardium, Skeletal muscle
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
50
issue
2
pages
7 pages
publisher
Informa Healthcare
external identifiers
  • scopus:0025260789
  • pmid:2339279
ISSN
0036-5513
DOI
10.3109/00365519009089147
language
English
LU publication?
no
id
b7240349-08ce-472e-93ac-e5a9c6deaf3b
date added to LUP
2016-08-30 22:06:47
date last changed
2024-01-04 11:47:53
@article{b7240349-08ce-472e-93ac-e5a9c6deaf3b,
  abstract     = {{<p>Cobalt has been shown to accumulate in the myocardium of uraemic patients and has been suggested as a myocardial toxin inhibiting mitochondrial respiration. In order to study the cellular effects of cobalt exposure three groups of rats (n=12 per group) were fed a diet containing 12% protein without supplementation or with 20 mg and 40 mg CoSO<sub>4</sub> 7 H<sub>2</sub>O/kg body weight/day respectively. After 8 weeks the hearts and soleus muscles were removed. Cobalt in tissues and in four cell fractions were analysed with neutron-activation analysis (ng/g wet weight and ng/mg protein respectively). Mitochondrial respiration was analysed as ATP-production rate using pyruvate+malate and palmitoyl-carnitine+malate as substrate. The ATP-production from pyruvate+ malate was unchanged in both heart and skeletal muscle in the exposed animals. With palmitate as substrate, the heart muscle showed a slightly lower ATP-production rate (p</p>}},
  author       = {{Clyne, N. and Wibom, R. and Havu, N. and Hultman, E. and Pehrsson, S. K. and Persson, B.}},
  issn         = {{0036-5513}},
  keywords     = {{Cobalt; Mitochondrial ATP-production; Rat myocardium; Skeletal muscle}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{153--159}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical & Laboratory Investigation}},
  title        = {{The effect of Cobalt on mitochondrial ATP-production in the rat myocardium and skeletal muscle}},
  url          = {{http://dx.doi.org/10.3109/00365519009089147}},
  doi          = {{10.3109/00365519009089147}},
  volume       = {{50}},
  year         = {{1990}},
}