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Animal-Friendly Affinity Reagents : Replacing the Needless in the Haystack

Gray, A. C.; Sidhu, S. S.; Chandrasekera, P. C.; Hendriksen, C. F M and Borrebaeck, C. A K LU (2016) In Trends in Biotechnology 34(12). p.960-969
Abstract

The multibillion-dollar global antibody industry produces an indispensable resource but that is generated using millions of animals. Despite the irrefutable maturation and availability of animal-friendly affinity reagents (AFAs) employing naïve B lymphocyte or synthetic recombinant technologies expressed by phage display, animal immunisation is still authorised for antibody production. Remarkably, replacement opportunities have been overlooked, despite the enormous potential reduction in animal use. Directive 2010/63/EU requires that animals are not used where alternatives exist. To ensure its implementation, we have engaged in discussions with the EU Reference Laboratory for alternatives to animal testing (EURL ECVAM) and the... (More)

The multibillion-dollar global antibody industry produces an indispensable resource but that is generated using millions of animals. Despite the irrefutable maturation and availability of animal-friendly affinity reagents (AFAs) employing naïve B lymphocyte or synthetic recombinant technologies expressed by phage display, animal immunisation is still authorised for antibody production. Remarkably, replacement opportunities have been overlooked, despite the enormous potential reduction in animal use. Directive 2010/63/EU requires that animals are not used where alternatives exist. To ensure its implementation, we have engaged in discussions with the EU Reference Laboratory for alternatives to animal testing (EURL ECVAM) and the Directorate General for Environment to carve out an EU-led replacement strategy. Measures must be imposed to avoid outsourcing, regulate commercial production, and ensure that antibody producers are fully supported.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3Rs, AFAs, alternatives to animals, animal welfare, animal-friendly affinity reagents, antibodies, Directive 2010/63/EU, monoclonal, polyclonal
in
Trends in Biotechnology
volume
34
issue
12
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:84979608737
  • wos:000389109300005
ISSN
0167-7799
DOI
10.1016/j.tibtech.2016.05.017
language
English
LU publication?
yes
id
b7611b64-3c52-4c86-8f4c-de4ff91f4010
date added to LUP
2016-12-29 12:33:42
date last changed
2017-09-18 11:34:11
@article{b7611b64-3c52-4c86-8f4c-de4ff91f4010,
  abstract     = {<p>The multibillion-dollar global antibody industry produces an indispensable resource but that is generated using millions of animals. Despite the irrefutable maturation and availability of animal-friendly affinity reagents (AFAs) employing naïve B lymphocyte or synthetic recombinant technologies expressed by phage display, animal immunisation is still authorised for antibody production. Remarkably, replacement opportunities have been overlooked, despite the enormous potential reduction in animal use. Directive 2010/63/EU requires that animals are not used where alternatives exist. To ensure its implementation, we have engaged in discussions with the EU Reference Laboratory for alternatives to animal testing (EURL ECVAM) and the Directorate General for Environment to carve out an EU-led replacement strategy. Measures must be imposed to avoid outsourcing, regulate commercial production, and ensure that antibody producers are fully supported.</p>},
  author       = {Gray, A. C. and Sidhu, S. S. and Chandrasekera, P. C. and Hendriksen, C. F M and Borrebaeck, C. A K},
  issn         = {0167-7799},
  keyword      = {3Rs,AFAs,alternatives to animals,animal welfare,animal-friendly affinity reagents,antibodies,Directive 2010/63/EU,monoclonal,polyclonal},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {960--969},
  publisher    = {Elsevier},
  series       = {Trends in Biotechnology},
  title        = {Animal-Friendly Affinity Reagents : Replacing the Needless in the Haystack},
  url          = {http://dx.doi.org/10.1016/j.tibtech.2016.05.017},
  volume       = {34},
  year         = {2016},
}