Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Lipid bilayer composition as a determinant of cancer cell sensitivity to tumoricidal protein-lipid complexes

Ho, James C.S. ; Mir, Sartaj Ahmad ; Cavalera, Michele LU ; Esmaeili, Parisa LU ; Tran, Tuan Hiep ; Yann, Zandra Chew ; Tran, Thi Hien LU ; Chaudhuri, Arunima LU ; Bendt, Anne K. and Wenk, Markus R. , et al. (2022) In BioFactors 48(5). p.1145-1159
Abstract

Complexes formed by the alpha1 N-terminal peptide of alpha-lactalbumin and oleic acid (alpha1-oleate) interact with lipid bilayers. Plasma membrane perturbations trigger tumor cell death but normal differentiated cells are more resistant, and their plasma membranes are less strongly affected. This study examined membrane lipid composition as a determinant of tumor cell reactivity. Bladder cancer tissue showed a higher abundance of unsaturated lipids enriched in phosphatidylcholine, PC (36:4) and PC (38:4), and sphingomyelin, SM (36:1) than healthy bladder tissue, where saturated lipids predominated and the lipid extracts from bladder cancer tissue inhibited the tumoricidal effect of the complex more effectively than healthy tissue... (More)

Complexes formed by the alpha1 N-terminal peptide of alpha-lactalbumin and oleic acid (alpha1-oleate) interact with lipid bilayers. Plasma membrane perturbations trigger tumor cell death but normal differentiated cells are more resistant, and their plasma membranes are less strongly affected. This study examined membrane lipid composition as a determinant of tumor cell reactivity. Bladder cancer tissue showed a higher abundance of unsaturated lipids enriched in phosphatidylcholine, PC (36:4) and PC (38:4), and sphingomyelin, SM (36:1) than healthy bladder tissue, where saturated lipids predominated and the lipid extracts from bladder cancer tissue inhibited the tumoricidal effect of the complex more effectively than healthy tissue extracts. Furthermore, unsaturated PC in solution inhibited tumor cell death, and the complex interacted with giant unilamellar vesicles formed by PC, confirming the affinity of alpha1-oleate for fluid membranes enriched in PC. Quartz Crystal Microbalance with dissipation monitoring (QCM-D) detected a preference of the complex for the liquid-disordered phase, suggesting that the insertion into PC-based membranes and the resulting membrane perturbations are influenced by membrane lipid saturation. The results suggest that the membrane lipid composition is functionally important and that specific unsaturated membrane lipids may serve as “recognition motifs” for broad-spectrum tumoricidal molecules such as alpha1-oleate.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acyl chain unsaturation, lipidomics, plasma membrane, protein-lipid complexes, tumor specificity
in
BioFactors
volume
48
issue
5
pages
1145 - 1159
publisher
Wiley
external identifiers
  • pmid:35388547
  • scopus:85127552287
ISSN
0951-6433
DOI
10.1002/biof.1841
language
English
LU publication?
yes
id
b7674d43-3996-4383-863c-9bfcc7a04a7f
date added to LUP
2022-06-03 15:03:06
date last changed
2024-04-18 12:38:42
@article{b7674d43-3996-4383-863c-9bfcc7a04a7f,
  abstract     = {{<p>Complexes formed by the alpha1 N-terminal peptide of alpha-lactalbumin and oleic acid (alpha1-oleate) interact with lipid bilayers. Plasma membrane perturbations trigger tumor cell death but normal differentiated cells are more resistant, and their plasma membranes are less strongly affected. This study examined membrane lipid composition as a determinant of tumor cell reactivity. Bladder cancer tissue showed a higher abundance of unsaturated lipids enriched in phosphatidylcholine, PC (36:4) and PC (38:4), and sphingomyelin, SM (36:1) than healthy bladder tissue, where saturated lipids predominated and the lipid extracts from bladder cancer tissue inhibited the tumoricidal effect of the complex more effectively than healthy tissue extracts. Furthermore, unsaturated PC in solution inhibited tumor cell death, and the complex interacted with giant unilamellar vesicles formed by PC, confirming the affinity of alpha1-oleate for fluid membranes enriched in PC. Quartz Crystal Microbalance with dissipation monitoring (QCM-D) detected a preference of the complex for the liquid-disordered phase, suggesting that the insertion into PC-based membranes and the resulting membrane perturbations are influenced by membrane lipid saturation. The results suggest that the membrane lipid composition is functionally important and that specific unsaturated membrane lipids may serve as “recognition motifs” for broad-spectrum tumoricidal molecules such as alpha1-oleate.</p>}},
  author       = {{Ho, James C.S. and Mir, Sartaj Ahmad and Cavalera, Michele and Esmaeili, Parisa and Tran, Tuan Hiep and Yann, Zandra Chew and Tran, Thi Hien and Chaudhuri, Arunima and Bendt, Anne K. and Wenk, Markus R. and Svanborg, Catharina}},
  issn         = {{0951-6433}},
  keywords     = {{acyl chain unsaturation; lipidomics; plasma membrane; protein-lipid complexes; tumor specificity}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1145--1159}},
  publisher    = {{Wiley}},
  series       = {{BioFactors}},
  title        = {{Lipid bilayer composition as a determinant of cancer cell sensitivity to tumoricidal protein-lipid complexes}},
  url          = {{http://dx.doi.org/10.1002/biof.1841}},
  doi          = {{10.1002/biof.1841}},
  volume       = {{48}},
  year         = {{2022}},
}