Thermophilic β-mannanases from bacteria : production, resources, structural features and bioengineering strategies
(2024) In World Journal of Microbiology and Biotechnology 40(4).- Abstract
β-mannanases are pivotal enzymes that cleave the mannan backbone to release short chain mannooligosaccharides, which have tremendous biotechnological applications including food/feed, prebiotics and biofuel production. Due to the high temperature conditions in many industrial applications, thermophilic mannanases seem to have great potential to overcome the thermal impediments. Thus, structural analysis of thermostable β-mannanases is extremely important, as it could open up new avenues for genetic engineering, and protein engineering of these enzymes with enhanced properties and catalytic efficiencies. Under this scope, the present review provides a state-of-the-art discussion on the thermophilic β-mannanases from bacterial origin,... (More)
β-mannanases are pivotal enzymes that cleave the mannan backbone to release short chain mannooligosaccharides, which have tremendous biotechnological applications including food/feed, prebiotics and biofuel production. Due to the high temperature conditions in many industrial applications, thermophilic mannanases seem to have great potential to overcome the thermal impediments. Thus, structural analysis of thermostable β-mannanases is extremely important, as it could open up new avenues for genetic engineering, and protein engineering of these enzymes with enhanced properties and catalytic efficiencies. Under this scope, the present review provides a state-of-the-art discussion on the thermophilic β-mannanases from bacterial origin, their production, engineering and structural characterization. It covers broad insights into various molecular biology techniques such as gene mutagenesis, heterologous gene expression, and protein engineering, that are employed to improve the catalytic efficiency and thermostability of bacterial mannanases for potential industrial applications. Further, the bottlenecks associated with mannanase production and process optimization are also discussed. Finally, future research related to bioengineering of mannanases with novel protein expression systems for commercial applications are also elaborated.
(Less)
- author
- Sadaqat, Beenish LU ; Dar, Mudasir A. ; Sha, Chong ; Abomohra, Abdelfatah ; Shao, Weilan and Yong, Yang Chun
- organization
- publishing date
- 2024-04
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacterial β-mannanases, Heat-shock induction, Mannan, Molecular modification, Thermophilic β-mannanase, Thermostability
- in
- World Journal of Microbiology and Biotechnology
- volume
- 40
- issue
- 4
- article number
- 130
- publisher
- Springer
- external identifiers
-
- pmid:38460032
- scopus:85187171575
- ISSN
- 0959-3993
- DOI
- 10.1007/s11274-024-03912-4
- language
- English
- LU publication?
- yes
- id
- b7823e6d-b594-42bd-9630-95c0f4f5f969
- date added to LUP
- 2024-04-03 09:26:00
- date last changed
- 2024-04-17 11:33:56
@article{b7823e6d-b594-42bd-9630-95c0f4f5f969,
abstract = {{<p>β-mannanases are pivotal enzymes that cleave the mannan backbone to release short chain mannooligosaccharides, which have tremendous biotechnological applications including food/feed, prebiotics and biofuel production. Due to the high temperature conditions in many industrial applications, thermophilic mannanases seem to have great potential to overcome the thermal impediments. Thus, structural analysis of thermostable β-mannanases is extremely important, as it could open up new avenues for genetic engineering, and protein engineering of these enzymes with enhanced properties and catalytic efficiencies. Under this scope, the present review provides a state-of-the-art discussion on the thermophilic β-mannanases from bacterial origin, their production, engineering and structural characterization. It covers broad insights into various molecular biology techniques such as gene mutagenesis, heterologous gene expression, and protein engineering, that are employed to improve the catalytic efficiency and thermostability of bacterial mannanases for potential industrial applications. Further, the bottlenecks associated with mannanase production and process optimization are also discussed. Finally, future research related to bioengineering of mannanases with novel protein expression systems for commercial applications are also elaborated.</p>}},
author = {{Sadaqat, Beenish and Dar, Mudasir A. and Sha, Chong and Abomohra, Abdelfatah and Shao, Weilan and Yong, Yang Chun}},
issn = {{0959-3993}},
keywords = {{Bacterial β-mannanases; Heat-shock induction; Mannan; Molecular modification; Thermophilic β-mannanase; Thermostability}},
language = {{eng}},
number = {{4}},
publisher = {{Springer}},
series = {{World Journal of Microbiology and Biotechnology}},
title = {{Thermophilic β-mannanases from bacteria : production, resources, structural features and bioengineering strategies}},
url = {{http://dx.doi.org/10.1007/s11274-024-03912-4}},
doi = {{10.1007/s11274-024-03912-4}},
volume = {{40}},
year = {{2024}},
}