A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications
(2013) In Aquacultural Engineering 55. p.23-31- Abstract
- In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry tanks (250 L in volume each) housed animals, and a tenth (without animals) acted as a reference tank. A single measurement unit made sequential measurements of each tank to eliminate the problem of sensor variation associated with multi-probe setups. The accuracy of the analyzers in relation to measurement range was: O-2 = 1%; CO2 < 1%; NH3 = 2%; temperature <= 0.25%; and pH +/- 0.01. Dissolved CO2 was measured using air-water... (More)
- In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry tanks (250 L in volume each) housed animals, and a tenth (without animals) acted as a reference tank. A single measurement unit made sequential measurements of each tank to eliminate the problem of sensor variation associated with multi-probe setups. The accuracy of the analyzers in relation to measurement range was: O-2 = 1%; CO2 < 1%; NH3 = 2%; temperature <= 0.25%; and pH +/- 0.01. Dissolved CO2 was measured using air-water equilibration coupled with non-dispersive infrared detection of carrier gas, and NH3 was quantified using a reagent-based assay and spectophotometric autoanalyzer. Though expensive and not common in aquaculture or physiology research, these two automated metabolite analyzers could operate in both fresh and seawater, and offered high precision and accuracy. We report on the performance of these instruments for aquaculture research in two trials using a freshwater (rainbow trout, Oncorhynchus mykiss) and seawater fish species (turbot, Scophthalmus maximus). One of the main constraints imposed by the sequential measurement of multiple tanks was the measurement frequency of each tank. In the aforementioned system, NH3 analyzes took the longest (12 min), followed by CO2 (7 min), O-2 (6 min), and pH (3 mm). (C) 2013 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3987186
- author
- Stiller, Kevin Torben ; Moran, Damian LU ; Vanselow, Klaus Heinrich ; Marxen, Kai ; Wuertz, Sven and Schulz, Carsten
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ammonia, Carbon dioxide, Oxygen, Respirometry, Recirculating aquaculture, system, Washout effect
- in
- Aquacultural Engineering
- volume
- 55
- pages
- 23 - 31
- publisher
- Elsevier
- external identifiers
-
- wos:000320216100004
- scopus:84878111155
- ISSN
- 1873-5614
- DOI
- 10.1016/j.aquaeng.2013.01.004
- language
- English
- LU publication?
- yes
- id
- b7dc57a4-2a03-4aec-b77b-f3018a1d170b (old id 3987186)
- date added to LUP
- 2016-04-01 10:17:06
- date last changed
- 2022-04-12 03:57:43
@article{b7dc57a4-2a03-4aec-b77b-f3018a1d170b,
abstract = {{In this study we describe a novel flow-through respirometer with automated and semi-continuous detection of key water variables. The recirculating aquaculture system was designed to house aquatic organisms in culture-like conditions and allow long-term, high-precision measurements. Nine respirometry tanks (250 L in volume each) housed animals, and a tenth (without animals) acted as a reference tank. A single measurement unit made sequential measurements of each tank to eliminate the problem of sensor variation associated with multi-probe setups. The accuracy of the analyzers in relation to measurement range was: O-2 = 1%; CO2 < 1%; NH3 = 2%; temperature <= 0.25%; and pH +/- 0.01. Dissolved CO2 was measured using air-water equilibration coupled with non-dispersive infrared detection of carrier gas, and NH3 was quantified using a reagent-based assay and spectophotometric autoanalyzer. Though expensive and not common in aquaculture or physiology research, these two automated metabolite analyzers could operate in both fresh and seawater, and offered high precision and accuracy. We report on the performance of these instruments for aquaculture research in two trials using a freshwater (rainbow trout, Oncorhynchus mykiss) and seawater fish species (turbot, Scophthalmus maximus). One of the main constraints imposed by the sequential measurement of multiple tanks was the measurement frequency of each tank. In the aforementioned system, NH3 analyzes took the longest (12 min), followed by CO2 (7 min), O-2 (6 min), and pH (3 mm). (C) 2013 Elsevier B.V. All rights reserved.}},
author = {{Stiller, Kevin Torben and Moran, Damian and Vanselow, Klaus Heinrich and Marxen, Kai and Wuertz, Sven and Schulz, Carsten}},
issn = {{1873-5614}},
keywords = {{Ammonia; Carbon dioxide; Oxygen; Respirometry; Recirculating aquaculture; system; Washout effect}},
language = {{eng}},
pages = {{23--31}},
publisher = {{Elsevier}},
series = {{Aquacultural Engineering}},
title = {{A novel respirometer for online detection of metabolites in aquaculture research: Evaluation and first applications}},
url = {{http://dx.doi.org/10.1016/j.aquaeng.2013.01.004}},
doi = {{10.1016/j.aquaeng.2013.01.004}},
volume = {{55}},
year = {{2013}},
}