The Kluyveromyces lactis gamma-toxin targets tRNA anticodons
(2005) In RNA 11(11). p.54-1648- Abstract
Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and... (More)
Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.
(Less)
- author
- Lu, Jian ; Huang, Bo ; Esberg, Anders ; Johansson, Marcus J O LU and Byström, Anders S
- publishing date
- 2005-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Anticodon/drug effects, Drug Resistance, Fungal/genetics, Gene Expression, Killer Factors, Yeast, Kluyveromyces/chemistry, Mutation/genetics, Mycotoxins/genetics, Nucleic Acid Conformation, RNA, Transfer/drug effects, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/physiology
- in
- RNA
- volume
- 11
- issue
- 11
- pages
- 54 - 1648
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- scopus:27144474752
- pmid:16244131
- ISSN
- 1355-8382
- DOI
- 10.1261/rna.2172105
- language
- English
- LU publication?
- no
- id
- b7f66d28-69fb-4427-8298-f40210b82aaa
- date added to LUP
- 2024-03-05 16:59:43
- date last changed
- 2024-06-13 12:39:46
@article{b7f66d28-69fb-4427-8298-f40210b82aaa, abstract = {{<p>Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.</p>}}, author = {{Lu, Jian and Huang, Bo and Esberg, Anders and Johansson, Marcus J O and Byström, Anders S}}, issn = {{1355-8382}}, keywords = {{Anticodon/drug effects; Drug Resistance, Fungal/genetics; Gene Expression; Killer Factors, Yeast; Kluyveromyces/chemistry; Mutation/genetics; Mycotoxins/genetics; Nucleic Acid Conformation; RNA, Transfer/drug effects; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/physiology}}, language = {{eng}}, number = {{11}}, pages = {{54--1648}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{RNA}}, title = {{The Kluyveromyces lactis gamma-toxin targets tRNA anticodons}}, url = {{http://dx.doi.org/10.1261/rna.2172105}}, doi = {{10.1261/rna.2172105}}, volume = {{11}}, year = {{2005}}, }