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The Kluyveromyces lactis gamma-toxin targets tRNA anticodons

Lu, Jian ; Huang, Bo ; Esberg, Anders ; Johansson, Marcus J O LU and Byström, Anders S (2005) In RNA 11(11). p.54-1648
Abstract

Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and... (More)

Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Anticodon/drug effects, Drug Resistance, Fungal/genetics, Gene Expression, Killer Factors, Yeast, Kluyveromyces/chemistry, Mutation/genetics, Mycotoxins/genetics, Nucleic Acid Conformation, RNA, Transfer/drug effects, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/physiology
in
RNA
volume
11
issue
11
pages
54 - 1648
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • scopus:27144474752
  • pmid:16244131
ISSN
1355-8382
DOI
10.1261/rna.2172105
language
English
LU publication?
no
id
b7f66d28-69fb-4427-8298-f40210b82aaa
date added to LUP
2024-03-05 16:59:43
date last changed
2024-06-13 12:39:46
@article{b7f66d28-69fb-4427-8298-f40210b82aaa,
  abstract     = {{<p>Kluyveromyces lactis killer strains secrete a heterotrimeric toxin (zymocin), which causes an irreversible growth arrest of sensitive yeast cells. Despite many efforts, the target(s) of the cytotoxic gamma-subunit of zymocin has remained elusive. Here we show that three tRNA species tRNA(Glu)(mcm(5)s(2)UUC), tRNA(Lys)(mcm(5)s(2)UUU), and tRNA(Gln)(mcm(5)s(2)UUG) are the targets of gamma-toxin. The toxin inhibits growth by cleaving these tRNAs at the 3' side of the modified wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U). Transfer RNA lacking a part of or the entire mcm(5) group is inefficiently cleaved by gamma-toxin, explaining the gamma-toxin resistance of the modification-deficient trm9, elp1-elp6, and kti11-kti13 mutants. The K. lactis gamma-toxin is the first eukaryotic toxin shown to target tRNA.</p>}},
  author       = {{Lu, Jian and Huang, Bo and Esberg, Anders and Johansson, Marcus J O and Byström, Anders S}},
  issn         = {{1355-8382}},
  keywords     = {{Anticodon/drug effects; Drug Resistance, Fungal/genetics; Gene Expression; Killer Factors, Yeast; Kluyveromyces/chemistry; Mutation/genetics; Mycotoxins/genetics; Nucleic Acid Conformation; RNA, Transfer/drug effects; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/physiology}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{54--1648}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{RNA}},
  title        = {{The Kluyveromyces lactis gamma-toxin targets tRNA anticodons}},
  url          = {{http://dx.doi.org/10.1261/rna.2172105}},
  doi          = {{10.1261/rna.2172105}},
  volume       = {{11}},
  year         = {{2005}},
}