Selective infection of E. coli as a function of a specific molecular interaction.
(2002) In Journal of Molecular Recognition 15(1). p.27-32- Abstract
- Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected,... (More)
- Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106655
- author
- Nilsson, Nina ; Karlsson, Fredrik LU ; Rakonjac, Jasna and Borrebaeck, Carl LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Protein Engineering, Peptide Library, Escherichia coli Infections/*microbiology, Bacteriophages/metabolism/*physiology, Escherichia coli/metabolism/*physiology, Support, Non-U.S. Gov't, U.S. Gov't, Non-P.H.S.
- in
- Journal of Molecular Recognition
- volume
- 15
- issue
- 1
- pages
- 27 - 32
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000174191600005
- scopus:0036006148
- pmid:11870919
- ISSN
- 1099-1352
- DOI
- 10.1002/jmr.557
- language
- English
- LU publication?
- yes
- id
- b7fae7de-7bd0-49e0-996e-44a98e795c6e (old id 106655)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11870919&dopt=Abstract
- date added to LUP
- 2016-04-01 12:03:02
- date last changed
- 2022-01-26 22:07:18
@article{b7fae7de-7bd0-49e0-996e-44a98e795c6e, abstract = {{Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries.}}, author = {{Nilsson, Nina and Karlsson, Fredrik and Rakonjac, Jasna and Borrebaeck, Carl}}, issn = {{1099-1352}}, keywords = {{Protein Engineering; Peptide Library; Escherichia coli Infections/*microbiology; Bacteriophages/metabolism/*physiology; Escherichia coli/metabolism/*physiology; Support; Non-U.S. Gov't; U.S. Gov't; Non-P.H.S.}}, language = {{eng}}, number = {{1}}, pages = {{27--32}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Molecular Recognition}}, title = {{Selective infection of E. coli as a function of a specific molecular interaction.}}, url = {{http://dx.doi.org/10.1002/jmr.557}}, doi = {{10.1002/jmr.557}}, volume = {{15}}, year = {{2002}}, }