Differentiation of Nerve Fibers Storing CGRP and CGRP Receptors in the Peripheral Trigeminovascular System.
Eftekhari, Sajedeh LU ; Warfvinge, Karin LU
; Blixt, Frank
LU
and Edvinsson, Lars
LU
(2013)
In Journal of Pain
14(11).
p.1289-1303
- Abstract
- Primary headaches such as migraine are postulated to involve the activation of sensory trigeminal pain neurons that innervate intracranial blood vessels and the dura mater. It is suggested that local activation of these sensory nerves may involve dural mast cells as one factor in local inflammation, causing sensitization of meningeal nociceptors. Immunofluorescence was used to study the detailed distribution of calcitonin gene-related peptide (CGRP) and its receptor components calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) in whole-mount rat dura mater and in human dural vessels. The relative distributions of CGRP, CLR, and RAMP1 were evaluated with respect to each other and in relationship to... (More)
- Primary headaches such as migraine are postulated to involve the activation of sensory trigeminal pain neurons that innervate intracranial blood vessels and the dura mater. It is suggested that local activation of these sensory nerves may involve dural mast cells as one factor in local inflammation, causing sensitization of meningeal nociceptors. Immunofluorescence was used to study the detailed distribution of calcitonin gene-related peptide (CGRP) and its receptor components calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) in whole-mount rat dura mater and in human dural vessels. The relative distributions of CGRP, CLR, and RAMP1 were evaluated with respect to each other and in relationship to mast cells, myelin, substance P, neuronal nitric oxide synthase, pituitary adenylate cyclase-activating polypeptide, and vasoactive intestinal peptide. CGRP expression was found in thin unmyelinated fibers, whereas CLR and RAMP1 were expressed in thicker myelinated fibers coexpressed with an A-fiber marker. CLR and RAMP1 immunoreactivity colocalized with mast cell tryptase in rodent; however, expression of both receptor components was not observed in human mast cells. Immunoreactive substance P fibers coexpressed CGRP, although neuronal nitric oxide synthase and vasoactive intestinal peptide expression was very limited, and these fibers were distinct from the CGRP-positive fibers. Few pituitary adenylate cyclase-activating polypeptide immunoreactive fibers occurred and some colocalized with CGRP. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4005521
- author
- Eftekhari, Sajedeh
LU
; Warfvinge, Karin
LU
; Blixt, Frank
LU
and Edvinsson, Lars
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Pain
- volume
- 14
- issue
- 11
- pages
- 1289 - 1303
- publisher
- Elsevier
- external identifiers
-
- wos:000327047900003
- pmid:23958278
- scopus:84887084591
- pmid:23958278
- ISSN
- 1526-5900
- DOI
- 10.1016/j.jpain.2013.03.010
- language
- English
- LU publication?
- yes
- id
- b82202ea-e075-4e5a-93e6-2290318982e1 (old id 4005521)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23958278?dopt=Abstract
- date added to LUP
- 2016-04-01 11:14:52
- date last changed
- 2024-02-22 20:04:40
@article{b82202ea-e075-4e5a-93e6-2290318982e1,
abstract = {{Primary headaches such as migraine are postulated to involve the activation of sensory trigeminal pain neurons that innervate intracranial blood vessels and the dura mater. It is suggested that local activation of these sensory nerves may involve dural mast cells as one factor in local inflammation, causing sensitization of meningeal nociceptors. Immunofluorescence was used to study the detailed distribution of calcitonin gene-related peptide (CGRP) and its receptor components calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) in whole-mount rat dura mater and in human dural vessels. The relative distributions of CGRP, CLR, and RAMP1 were evaluated with respect to each other and in relationship to mast cells, myelin, substance P, neuronal nitric oxide synthase, pituitary adenylate cyclase-activating polypeptide, and vasoactive intestinal peptide. CGRP expression was found in thin unmyelinated fibers, whereas CLR and RAMP1 were expressed in thicker myelinated fibers coexpressed with an A-fiber marker. CLR and RAMP1 immunoreactivity colocalized with mast cell tryptase in rodent; however, expression of both receptor components was not observed in human mast cells. Immunoreactive substance P fibers coexpressed CGRP, although neuronal nitric oxide synthase and vasoactive intestinal peptide expression was very limited, and these fibers were distinct from the CGRP-positive fibers. Few pituitary adenylate cyclase-activating polypeptide immunoreactive fibers occurred and some colocalized with CGRP.}},
author = {{Eftekhari, Sajedeh and Warfvinge, Karin and Blixt, Frank and Edvinsson, Lars}},
issn = {{1526-5900}},
language = {{eng}},
number = {{11}},
pages = {{1289--1303}},
publisher = {{Elsevier}},
series = {{Journal of Pain}},
title = {{Differentiation of Nerve Fibers Storing CGRP and CGRP Receptors in the Peripheral Trigeminovascular System.}},
url = {{http://dx.doi.org/10.1016/j.jpain.2013.03.010}},
doi = {{10.1016/j.jpain.2013.03.010}},
volume = {{14}},
year = {{2013}},
}