Is acetylcholine a signaling molecule for human colon cancer progression?
(2011) In Scandinavian Journal of Gastroenterology 46(4). p.446-455- Abstract
- Objective. Non-neuronal acetylcholine (ACh) has been suggested to be a mediator for the development of various types of cancer. We analyzed a possible role for this molecule in carcinogenesis and/or progression of human colon cancer, in patient biopsies harvested from the colon during surgery. We addressed whether ACh synthesis (by choline acetyltransferase) and/or degradation (by ACh esterase), as well as the expression of the α7-subtype of the nicotinic ACh receptors, and the peptide ligand at the α7 receptors, secreted mammalian Ly6/urokinase-type plasminogen activator receptor-related protein-1, respectively, are deranged in tumor tissue as compared with macroscopically tumor-free colon tissue. Methods. A total of 38 patients were... (More)
- Objective. Non-neuronal acetylcholine (ACh) has been suggested to be a mediator for the development of various types of cancer. We analyzed a possible role for this molecule in carcinogenesis and/or progression of human colon cancer, in patient biopsies harvested from the colon during surgery. We addressed whether ACh synthesis (by choline acetyltransferase) and/or degradation (by ACh esterase), as well as the expression of the α7-subtype of the nicotinic ACh receptors, and the peptide ligand at the α7 receptors, secreted mammalian Ly6/urokinase-type plasminogen activator receptor-related protein-1, respectively, are deranged in tumor tissue as compared with macroscopically tumor-free colon tissue. Methods. A total of 38 patients were grouped for analysis based on their respective Dukes stage (either Dukes A + B or C + D). A mucosal tissue sample was harvested from macroscopically tumor-free colon tissue (i.e. control tissue), as well as from the tumor, and protein lysates were prepared for quantitative Western blotting. Full-thickness specimens were taken for immunohistochemistry. Results. For all the above named markers, there was a significant difference between control and tumor tissue with regard to protein levels, and there was, in addition, a significant difference in protein levels between the Dukes A + B and C + D groups. Conclusion. The current findings may suggest a role for ACh in colon carcinogenesis/cancer progression; the data obtained could have prognostic and/or therapeutic significance for this disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/b82d4eaa-8cd3-4a7a-bd42-71f8d1cae565
- author
- Novotny, Ann ; Ryberg, Kristin ; Heiman Ullmark, Jenny ; Nilsson, Linn LU ; Khorram-Manesh, Amir ; Nordgren, Svante ; Delbro, Dick and Nylund, Gunnar
- publishing date
- 2011-01-26
- type
- Contribution to journal
- publication status
- published
- in
- Scandinavian Journal of Gastroenterology
- volume
- 46
- issue
- 4
- pages
- 446 - 455
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:79952600431
- ISSN
- 0036-5521
- DOI
- 10.3109/00365521.2010.539252
- language
- English
- LU publication?
- no
- id
- b82d4eaa-8cd3-4a7a-bd42-71f8d1cae565
- date added to LUP
- 2024-05-29 15:17:42
- date last changed
- 2024-05-30 07:37:48
@article{b82d4eaa-8cd3-4a7a-bd42-71f8d1cae565,
abstract = {{Objective. Non-neuronal acetylcholine (ACh) has been suggested to be a mediator for the development of various types of cancer. We analyzed a possible role for this molecule in carcinogenesis and/or progression of human colon cancer, in patient biopsies harvested from the colon during surgery. We addressed whether ACh synthesis (by choline acetyltransferase) and/or degradation (by ACh esterase), as well as the expression of the α7-subtype of the nicotinic ACh receptors, and the peptide ligand at the α7 receptors, secreted mammalian Ly6/urokinase-type plasminogen activator receptor-related protein-1, respectively, are deranged in tumor tissue as compared with macroscopically tumor-free colon tissue. Methods. A total of 38 patients were grouped for analysis based on their respective Dukes stage (either Dukes A + B or C + D). A mucosal tissue sample was harvested from macroscopically tumor-free colon tissue (i.e. control tissue), as well as from the tumor, and protein lysates were prepared for quantitative Western blotting. Full-thickness specimens were taken for immunohistochemistry. Results. For all the above named markers, there was a significant difference between control and tumor tissue with regard to protein levels, and there was, in addition, a significant difference in protein levels between the Dukes A + B and C + D groups. Conclusion. The current findings may suggest a role for ACh in colon carcinogenesis/cancer progression; the data obtained could have prognostic and/or therapeutic significance for this disease.}},
author = {{Novotny, Ann and Ryberg, Kristin and Heiman Ullmark, Jenny and Nilsson, Linn and Khorram-Manesh, Amir and Nordgren, Svante and Delbro, Dick and Nylund, Gunnar}},
issn = {{0036-5521}},
language = {{eng}},
month = {{01}},
number = {{4}},
pages = {{446--455}},
publisher = {{Taylor & Francis}},
series = {{Scandinavian Journal of Gastroenterology}},
title = {{Is acetylcholine a signaling molecule for human colon cancer progression?}},
url = {{http://dx.doi.org/10.3109/00365521.2010.539252}},
doi = {{10.3109/00365521.2010.539252}},
volume = {{46}},
year = {{2011}},
}