Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profiling
(2021) In International Journal of Molecular Sciences 22(21).- Abstract
Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field... (More)
Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
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- author
- Abreha, Kibrom B. ; Alexandersson, Erik LU ; Resjö, Svante LU ; Lankinen, Åsa LU ; Sueldo, Daniela ; Kaschani, Farnusch ; Kaiser, Markus ; van der Hoorn, Renier A.L. ; Levander, Fredrik LU and Andreasson, Erik LU
- organization
- publishing date
- 2021-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ABPP, Apoplast, Field-omics, Potato, Proteomics, Serine hydrolases, β-glycosidases
- in
- International Journal of Molecular Sciences
- volume
- 22
- issue
- 21
- article number
- 12033
- publisher
- MDPI AG
- external identifiers
-
- scopus:85118566610
- pmid:34769464
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms222112033
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- b856f67c-c999-48aa-ba29-18aa17701573
- date added to LUP
- 2021-11-24 10:54:15
- date last changed
- 2024-04-20 16:25:43
@article{b856f67c-c999-48aa-ba29-18aa17701573,
abstract = {{<p>Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June–August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June–August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July–August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.</p>}},
author = {{Abreha, Kibrom B. and Alexandersson, Erik and Resjö, Svante and Lankinen, Åsa and Sueldo, Daniela and Kaschani, Farnusch and Kaiser, Markus and van der Hoorn, Renier A.L. and Levander, Fredrik and Andreasson, Erik}},
issn = {{1661-6596}},
keywords = {{ABPP; Apoplast; Field-omics; Potato; Proteomics; Serine hydrolases; β-glycosidases}},
language = {{eng}},
month = {{11}},
number = {{21}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Article leaf apoplast of field-grown potato analyzed by quantitative proteomics and activity-based protein profiling}},
url = {{http://dx.doi.org/10.3390/ijms222112033}},
doi = {{10.3390/ijms222112033}},
volume = {{22}},
year = {{2021}},
}