Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy
(2020) In Scientific Reports 10(1). p.1942-1942- Abstract
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
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https://lup.lub.lu.se/record/b864eaab-d40d-4d40-aaab-fd35b686cb4f
- author
- publishing date
- 2020-02-06
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- pages
- 1942 - 1942
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:32029815
- scopus:85079063271
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-019-54349-x
- language
- English
- LU publication?
- no
- id
- b864eaab-d40d-4d40-aaab-fd35b686cb4f
- date added to LUP
- 2020-09-09 18:28:28
- date last changed
- 2024-09-05 04:20:55
@article{b864eaab-d40d-4d40-aaab-fd35b686cb4f, abstract = {{<p>Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.</p>}}, author = {{de Medeiros, Gustavo and Kromm, Dimitri and Balazs, Balint and Norlin, Nils and Günther, Stefan and Izquierdo, Emiliano and Ronchi, Paolo and Komoto, Shinya and Krzic, Uros and Schwab, Yannick and Peri, Francesca and de Renzis, Stefano and Leptin, Maria and Rauzi, Matteo and Hufnagel, Lars}}, issn = {{2045-2322}}, language = {{eng}}, month = {{02}}, number = {{1}}, pages = {{1942--1942}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy}}, url = {{http://dx.doi.org/10.1038/s41598-019-54349-x}}, doi = {{10.1038/s41598-019-54349-x}}, volume = {{10}}, year = {{2020}}, }