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Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

de Medeiros, Gustavo ; Kromm, Dimitri ; Balazs, Balint ; Norlin, Nils LU ; Günther, Stefan ; Izquierdo, Emiliano ; Ronchi, Paolo ; Komoto, Shinya ; Krzic, Uros and Schwab, Yannick , et al. (2020) In Scientific Reports 10(1). p.1942-1942
Abstract

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

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publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
10
issue
1
pages
1942 - 1942
publisher
Nature Publishing Group
external identifiers
  • pmid:32029815
  • scopus:85079063271
ISSN
2045-2322
DOI
10.1038/s41598-019-54349-x
language
English
LU publication?
no
id
b864eaab-d40d-4d40-aaab-fd35b686cb4f
date added to LUP
2020-09-09 18:28:28
date last changed
2024-06-12 20:16:39
@article{b864eaab-d40d-4d40-aaab-fd35b686cb4f,
  abstract     = {{<p>Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.</p>}},
  author       = {{de Medeiros, Gustavo and Kromm, Dimitri and Balazs, Balint and Norlin, Nils and Günther, Stefan and Izquierdo, Emiliano and Ronchi, Paolo and Komoto, Shinya and Krzic, Uros and Schwab, Yannick and Peri, Francesca and de Renzis, Stefano and Leptin, Maria and Rauzi, Matteo and Hufnagel, Lars}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{1}},
  pages        = {{1942--1942}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy}},
  url          = {{http://dx.doi.org/10.1038/s41598-019-54349-x}},
  doi          = {{10.1038/s41598-019-54349-x}},
  volume       = {{10}},
  year         = {{2020}},
}