Continuous superporous agarose beds in radial flow columns
(2001) In Journal of chromatography. A 925(1-2). p.69-78- Abstract
- Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied in the range of 10–100 μm. In this work, large diameter beds (60 mm) were prepared and used in specially designed radial flow columns. The basic chromatographic properties of the beds were investigated by size-exclusion chromatography experiments. In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract. In a biotransformation application one bed was provided with... (More)
- Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied in the range of 10–100 μm. In this work, large diameter beds (60 mm) were prepared and used in specially designed radial flow columns. The basic chromatographic properties of the beds were investigated by size-exclusion chromatography experiments. In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract. In a biotransformation application one bed was provided with immobilized β-galactosidase and used in the production of lactose-free milk.
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Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/b89f4878-7779-4281-8f61-0a30fd63c007
- author
- Gustavsson, Per-Erik LU and Larsson, Per-Olof LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- monolithic chromatography, superporous agarose, affinity chromatography, enzyme reactor
- in
- Journal of chromatography. A
- volume
- 925
- issue
- 1-2
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0035800678
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(01)01027-5
- language
- English
- LU publication?
- yes
- id
- b89f4878-7779-4281-8f61-0a30fd63c007
- date added to LUP
- 2024-05-16 15:33:46
- date last changed
- 2024-05-20 20:58:00
@article{b89f4878-7779-4281-8f61-0a30fd63c007, abstract = {{Continuous superporous agarose beds constitute a new support material for chromatography, biocatalysis and electrophoresis. The bed consists of a single piece of agarose gel, homogeneously transected by flow-carrying pores, which easily can be varied in the range of 10–100 μm. In this work, large diameter beds (60 mm) were prepared and used in specially designed radial flow columns. The basic chromatographic properties of the beds were investigated by size-exclusion chromatography experiments. In an affinity chromatography application one bed was derivatized with Cibacron Blue 3GA and used for the purification of lactate dehydrogenase from a crude bovine heart extract. In a biotransformation application one bed was provided with immobilized β-galactosidase and used in the production of lactose-free milk.<br/>}}, author = {{Gustavsson, Per-Erik and Larsson, Per-Olof}}, issn = {{0021-9673}}, keywords = {{monolithic chromatography; superporous agarose; affinity chromatography; enzyme reactor}}, language = {{eng}}, number = {{1-2}}, pages = {{69--78}}, publisher = {{Elsevier}}, series = {{Journal of chromatography. A}}, title = {{Continuous superporous agarose beds in radial flow columns}}, url = {{http://dx.doi.org/10.1016/S0021-9673(01)01027-5}}, doi = {{10.1016/S0021-9673(01)01027-5}}, volume = {{925}}, year = {{2001}}, }