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Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-injection of Patient-Derived T Cells in Immune-Deficient NSG Mice

Gunnarsdóttir, Fríða Björk LU ; Kiessling, Rolf and Pico de Coaña, Yago (2019) In Methods in molecular biology (Clifton, N.J.) 1913. p.207-215
Abstract

When primary tumor cells are grown in vitro, they are exposed to an environment that is vastly different from the tumor environment they originate from. The in vitro environment can lack the three-dimensional structure of the tumor, other cell types present within the tumor microenvironment, and important growth factors. Humanized mouse models allow researchers to study primary tumor cells in a more natural environment. With further development of several strains of immune-deficient mice, the mouse model allows for observation of the patient-derived tumor xenograft (PDTX) growth alone as well as in the presence of a human immune system. We describe how this can be accomplished with injection of single cell suspension of melanoma tumor... (More)

When primary tumor cells are grown in vitro, they are exposed to an environment that is vastly different from the tumor environment they originate from. The in vitro environment can lack the three-dimensional structure of the tumor, other cell types present within the tumor microenvironment, and important growth factors. Humanized mouse models allow researchers to study primary tumor cells in a more natural environment. With further development of several strains of immune-deficient mice, the mouse model allows for observation of the patient-derived tumor xenograft (PDTX) growth alone as well as in the presence of a human immune system. We describe how this can be accomplished with injection of single cell suspension of melanoma tumor cells into immune-deficient NOD-scid IL2Rγnull (NSG) mice. We also describe how tumor cells and immune cells can be co-injected, using Winn assay, and the possibility to use that method to study immune therapies for cancer.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immune deficiency, Melanoma, NSG mice, Tumor-infiltrating lymphocytes, Xenograft
in
Methods in molecular biology (Clifton, N.J.)
volume
1913
pages
9 pages
publisher
Springer
external identifiers
  • scopus:85060248952
ISSN
1940-6029
DOI
10.1007/978-1-4939-8979-9_15
language
English
LU publication?
yes
id
b8b123c3-42ab-4335-b1f8-e6baaca836d1
date added to LUP
2019-01-30 09:42:37
date last changed
2019-02-27 05:11:25
@article{b8b123c3-42ab-4335-b1f8-e6baaca836d1,
  abstract     = {<p>When primary tumor cells are grown in vitro, they are exposed to an environment that is vastly different from the tumor environment they originate from. The in vitro environment can lack the three-dimensional structure of the tumor, other cell types present within the tumor microenvironment, and important growth factors. Humanized mouse models allow researchers to study primary tumor cells in a more natural environment. With further development of several strains of immune-deficient mice, the mouse model allows for observation of the patient-derived tumor xenograft (PDTX) growth alone as well as in the presence of a human immune system. We describe how this can be accomplished with injection of single cell suspension of melanoma tumor cells into immune-deficient NOD-scid IL2Rγnull (NSG) mice. We also describe how tumor cells and immune cells can be co-injected, using Winn assay, and the possibility to use that method to study immune therapies for cancer.</p>},
  author       = {Gunnarsdóttir, Fríða Björk and Kiessling, Rolf and Pico de Coaña, Yago},
  issn         = {1940-6029},
  keyword      = {Immune deficiency,Melanoma,NSG mice,Tumor-infiltrating lymphocytes,Xenograft},
  language     = {eng},
  pages        = {207--215},
  publisher    = {Springer},
  series       = {Methods in molecular biology (Clifton, N.J.)},
  title        = {Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-injection of Patient-Derived T Cells in Immune-Deficient NSG Mice},
  url          = {http://dx.doi.org/10.1007/978-1-4939-8979-9_15},
  volume       = {1913},
  year         = {2019},
}