Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy.
(2013) In Journal of Biomedical Optics 18(3).- Abstract
- We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from... (More)
- We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3628674
- author
- Merdasa, Aboma LU ; Brydegaard, Mikkel LU ; Svanberg, Sune LU and Zoueu, Jeremie T
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biomedical Optics
- volume
- 18
- issue
- 3
- article number
- 036002
- publisher
- SPIE
- external identifiers
-
- wos:000322336700022
- pmid:23455960
- scopus:84885985667
- pmid:23455960
- ISSN
- 1083-3668
- DOI
- 10.1117/1.JBO.18.3.036002
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Atomic physics (011013005), Chemical Physics (S) (011001060)
- id
- b8da6f16-b00d-45eb-bc32-4796d71e7ab0 (old id 3628674)
- date added to LUP
- 2016-04-01 10:51:31
- date last changed
- 2022-03-20 00:37:05
@article{b8da6f16-b00d-45eb-bc32-4796d71e7ab0,
abstract = {{We report an accurate optical differentiation technique between healthy and malaria-infected erythrocytes by quasi-simultaneous measurements of transmittance, reflectance, and scattering properties of unstained blood smears using a multispectral and multimode light-emitting diode microscope. We propose a technique for automated imaging, identification, and counting of malaria-infected erythrocytes for real-time and cost-effective parasitaemia diagnosis as an effective alternative to the manual screening of stained blood smears, now considered to be the gold standard in malaria diagnosis. We evaluate the performance of our algorithm against manual estimations of an expert and show a spectrally resolved increased scattering from malaria-infected blood cells.}},
author = {{Merdasa, Aboma and Brydegaard, Mikkel and Svanberg, Sune and Zoueu, Jeremie T}},
issn = {{1083-3668}},
language = {{eng}},
number = {{3}},
publisher = {{SPIE}},
series = {{Journal of Biomedical Optics}},
title = {{Staining-free malaria diagnostics by multispectral and multimodality light-emitting-diode microscopy.}},
url = {{http://dx.doi.org/10.1117/1.JBO.18.3.036002}},
doi = {{10.1117/1.JBO.18.3.036002}},
volume = {{18}},
year = {{2013}},
}