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Inhibitory phosphorylation of Cdk1 mediates prolonged prophase I arrest in female germ cells and is essential for female reproductive lifespan

Adhikari, Deepak ; Busayavalasa, Kiran ; Zhang, Jingjing ; Hu, Mengwen ; Risal, Sanjiv ; Bayazit, Mustafa Bilal ; Singh, Meenakshi ; Diril, M. Kasim ; Kaldis, Philipp LU and Liu, Kui (2016) In Cell Research 26(11). p.1212-1225
Abstract

A unique feature of female germ cell development in mammals is their remarkably long arrest at the prophase of meiosis I, which lasts up to 50 years in humans. Both dormant and growing oocytes are arrested at prophase I and completely lack the ability to resume meiosis. Here, we show that the prolonged meiotic arrest of female germ cells is largely achieved via the inhibitory phosphorylation of Cdk1 (cyclin-dependent kinase 1). In two mouse models where we have introduced mutant Cdk1 T14AY15F which cannot be inhibited by phosphorylation (Cdk1AF) in small meiotically incompetent oocytes, the prophase I arrest is interrupted, leading to a premature loss of female germ cells. We show that in growing oocytes, Cdk1AF leads to premature... (More)

A unique feature of female germ cell development in mammals is their remarkably long arrest at the prophase of meiosis I, which lasts up to 50 years in humans. Both dormant and growing oocytes are arrested at prophase I and completely lack the ability to resume meiosis. Here, we show that the prolonged meiotic arrest of female germ cells is largely achieved via the inhibitory phosphorylation of Cdk1 (cyclin-dependent kinase 1). In two mouse models where we have introduced mutant Cdk1 T14AY15F which cannot be inhibited by phosphorylation (Cdk1AF) in small meiotically incompetent oocytes, the prophase I arrest is interrupted, leading to a premature loss of female germ cells. We show that in growing oocytes, Cdk1AF leads to premature resumption of meiosis with condensed chromosomes and germinal vesicle breakdown followed by oocyte death, whereas in dormant oocytes, Cdk1AF leads to oocyte death directly, and both situations damage the ovarian reserve that maintains the female reproductive lifespan, which should be around 1 year in mice. Furthermore, interruption of the inhibitory phosphorylation of Cdk1 results in DNA damage, which is accompanied by induction of the Chk2 (checkpoint kinase 2)-p53/p63-dependent cell death pathway, which eventually causes global oocyte death. Together, our data demonstrate that the phosphorylation-mediated suppression of Cdk1 activity is one of the crucial factors that maintain the lengthy prophase arrest in mammalian female germ cells, which is essential for preserving the germ cell pool and reproductive lifespan in female mammals.

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author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cdk1 inhibitory phosphorylation, female fertility lifespan, immature oocyte, meiotic prophase I
in
Cell Research
volume
26
issue
11
pages
1212 - 1225
publisher
Science Press
external identifiers
  • scopus:84992111018
  • pmid:27767095
ISSN
1001-0602
DOI
10.1038/cr.2016.119
language
English
LU publication?
no
id
b8e1b7d9-0310-4ba7-9dfb-e27dc183c89b
date added to LUP
2019-09-18 13:45:41
date last changed
2021-04-18 05:18:04
@article{b8e1b7d9-0310-4ba7-9dfb-e27dc183c89b,
  abstract     = {<p>A unique feature of female germ cell development in mammals is their remarkably long arrest at the prophase of meiosis I, which lasts up to 50 years in humans. Both dormant and growing oocytes are arrested at prophase I and completely lack the ability to resume meiosis. Here, we show that the prolonged meiotic arrest of female germ cells is largely achieved via the inhibitory phosphorylation of Cdk1 (cyclin-dependent kinase 1). In two mouse models where we have introduced mutant Cdk1 T14AY15F which cannot be inhibited by phosphorylation (Cdk1AF) in small meiotically incompetent oocytes, the prophase I arrest is interrupted, leading to a premature loss of female germ cells. We show that in growing oocytes, Cdk1AF leads to premature resumption of meiosis with condensed chromosomes and germinal vesicle breakdown followed by oocyte death, whereas in dormant oocytes, Cdk1AF leads to oocyte death directly, and both situations damage the ovarian reserve that maintains the female reproductive lifespan, which should be around 1 year in mice. Furthermore, interruption of the inhibitory phosphorylation of Cdk1 results in DNA damage, which is accompanied by induction of the Chk2 (checkpoint kinase 2)-p53/p63-dependent cell death pathway, which eventually causes global oocyte death. Together, our data demonstrate that the phosphorylation-mediated suppression of Cdk1 activity is one of the crucial factors that maintain the lengthy prophase arrest in mammalian female germ cells, which is essential for preserving the germ cell pool and reproductive lifespan in female mammals.</p>},
  author       = {Adhikari, Deepak and Busayavalasa, Kiran and Zhang, Jingjing and Hu, Mengwen and Risal, Sanjiv and Bayazit, Mustafa Bilal and Singh, Meenakshi and Diril, M. Kasim and Kaldis, Philipp and Liu, Kui},
  issn         = {1001-0602},
  language     = {eng},
  month        = {10},
  number       = {11},
  pages        = {1212--1225},
  publisher    = {Science Press},
  series       = {Cell Research},
  title        = {Inhibitory phosphorylation of Cdk1 mediates prolonged prophase I arrest in female germ cells and is essential for female reproductive lifespan},
  url          = {http://dx.doi.org/10.1038/cr.2016.119},
  doi          = {10.1038/cr.2016.119},
  volume       = {26},
  year         = {2016},
}