Redox hydrogel-based bienzyme microelectrodes for amperometric monitoring of L-glutamate
(2002) In Electroanalysis 14(6). p.393-399- Abstract
- Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological... (More)
- Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological samples. The L-glutamate microsensors are characterized by a good operation stability and sensitivity (0.038+/-0.005 mAM(-1)), a low detection limit (0.5 muM in a conventional amperometric set-up and 0.03 muM in a Faraday cage, defined as three times the signal-to-noise ratio), a linear range up to 50 muM and a response time of about 35 s. The glutamate biosensors have been applied for the direct measurement Of L-glutamate release (upon chemical stimulation) from a population of immortalized hippocampal neurons (HN10 cells) demonstrating the possibility to amperometrically monitor in-Situ L-glutamate secretion from these cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/340844
- author
- Mikeladze, E ; Schulte, A ; Mosbach, M ; Blochl, A ; Csöregi, Elisabeth LU ; Solomonia, R and Schumann, W
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- hippocampal neurons, glutamate secretion, microelectrode, glutamate, amperometric biosensor
- in
- Electroanalysis
- volume
- 14
- issue
- 6
- pages
- 393 - 399
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000174832900001
- scopus:0036107897
- ISSN
- 1040-0397
- DOI
- 10.1002/1521-4109(200203)14:6<393::AID-ELAN393>3.0.CO;2-P
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Chemistry (011001220), Analytical Chemistry (S/LTH) (011001004)
- id
- b8f8b93b-2b01-4b06-89c5-291f76a2a522 (old id 340844)
- date added to LUP
- 2016-04-01 17:07:02
- date last changed
- 2022-03-07 18:37:30
@article{b8f8b93b-2b01-4b06-89c5-291f76a2a522,
abstract = {{Fabrication and characterization of amperometric bienzyme L-glutamate sensitive microelectrodes are the prerequisite for monitoring changes Of L-glutamate concentration at glutamate-secreting cell cultures. The design of the glutamate microelectrodes is based on incorporating L-glutamate oxidase and horseradish peroxidase into a redox-hydrogel containing PVI19-dmeOs as the redox mediator and immobilizing this system onto the surface of platinum microdisk electrodes using, a dip-coating procedure. For amperometric measurements Of L-glutamate, these redox hydrogel-based bienzyme microelectrodes can be operated at low working potentials (-50 mV vs. Ag/AgCl) decreasing the influence of electroactive interferants possibly present in biological samples. The L-glutamate microsensors are characterized by a good operation stability and sensitivity (0.038+/-0.005 mAM(-1)), a low detection limit (0.5 muM in a conventional amperometric set-up and 0.03 muM in a Faraday cage, defined as three times the signal-to-noise ratio), a linear range up to 50 muM and a response time of about 35 s. The glutamate biosensors have been applied for the direct measurement Of L-glutamate release (upon chemical stimulation) from a population of immortalized hippocampal neurons (HN10 cells) demonstrating the possibility to amperometrically monitor in-Situ L-glutamate secretion from these cells.}},
author = {{Mikeladze, E and Schulte, A and Mosbach, M and Blochl, A and Csöregi, Elisabeth and Solomonia, R and Schumann, W}},
issn = {{1040-0397}},
keywords = {{hippocampal neurons; glutamate secretion; microelectrode; glutamate; amperometric biosensor}},
language = {{eng}},
number = {{6}},
pages = {{393--399}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Electroanalysis}},
title = {{Redox hydrogel-based bienzyme microelectrodes for amperometric monitoring of L-glutamate}},
url = {{http://dx.doi.org/10.1002/1521-4109(200203)14:6<393::AID-ELAN393>3.0.CO;2-P}},
doi = {{10.1002/1521-4109(200203)14:6<393::AID-ELAN393>3.0.CO;2-P}},
volume = {{14}},
year = {{2002}},
}